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Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction


, : Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5(4): 874-879

We report a rapid and sensitive method for the detection of base changes in given sequences of genomic DNA. This technique is based on the facts that specific regions of genomic sequences can be efficently labeled and amplified simultaneously by using labeled substrates in the polymerase chain reaction and that in nondenaturing polyacrylamide gels, the electrophoretic mobility of single-stranded nucleic acid depends not only on its size but also on its sequence. The process does not involve restriction enzyme digestion, blotting, or hybridization to probes. We found that most single base changes in up to 200-base fragments could be detected as mobility shifts. RAS oncogene activation was detected by this technique. We also show that the interspersed repetitive sequences of human, Alu repeats are highly polymorphic.

US$29.90

PMID: 2687159


Other references

Whelan, RJ.; Langedyk, W.; Pashby, AS., 1980: The effects of wildfire on arthropod populations in Jarrah-Banksia woodland. The response of arthropods to fire within the fortnight immediately following burning was investigated. Arthropods were sampled in pitfall traps and by hand-searching in potential refuges in both burnt and unburnt areas. There were more individual...

Guenther, Konrad., 1912: Vom Urtier zum Menschen. Ein Bilderatlas zur Abstammungs- und Entwicklungsgeschichte des Menschen. 2. Aufl. 2 Bde. Unknown

Anonymous, 1973: Researchers offer some do's and don'ts when positioning oral surgery patients. Dental Survey 49(5): 37-37

Singh; Sengupta; Ismail; Jain, 1989: Long-range correlations in nucleus-nucleus interactions at ultrahigh energies. Physical Review. C, Nuclear Physics 39(5): 1835-1839

Takahashi, S.; Tanabe, K.; Maseda, C.; Shiono, H.; Fukui, Y., 1988: Increased plasma free fatty acid and triglyceride levels after single administration of toluene in rabbits. Changes of plasma lipids (triglyceride, TC: total cholesterol, Cho; and phospholipids, PL), free fatty acid (FFA), and blood glucose (BG) were studied in male rabbits after toluene [an industrial solvent] administration (0.5 g/kg per os). Hypertri...

de Carvalho, M., 2012: Estimating the value of estimation. Clinical Neurophysiology 123(10): 1904-1905

Dippold, W.; Kaulen, H.; Schwaeble, W.; Kerlin, M.; Meyer Zum Bueschenfelde, K.H., 1992: Growth factors, cytokines and adhesion molecules in pancreas carcinoma cells. Digestion 52(2): 78

Wada, Y.; Wisser, D.; Eisner, S.; Flörke, M.; Gerten, D.; Haddeland, I.; Hanasaki, N.; Masaki, Y.; Portmann, F.T.; Stacke, T.; Tessler, Z.; Schewe, J., 2013: Multi-model projections and uncertainties of irrigation water demand under climate change. Crop irrigation is responsible for 70% of humanity's water demand. Since the late 1990s, the expansion of irrigated areas has been tapering off, and this trend is expected to continue in the future. Future irrigation water demand (IWD) is, ho...

Schild, W.; Schurholz, K.; Seuken, A., 1963: Studies on the estrogen content of the umbilical cord vessels and of retroplacental blood. Archiv für Gynakologie 198: 136-139

Lucchi L.; Malmusi G.; Cappelli G.; Lusvarghi E., 1990: Heparin free predilution hemodiafiltration in patients at risk of bleeding. Kidney International 37(1): 308