+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Reaction of the cell center to exposure to the calcium ionophore A-23187

, : Reaction of the cell center to exposure to the calcium ionophore A-23187. Tsitologiia 31(3): 259-266

Calcium ionophore A23187, taken at a concentration of 0.1 microgram/ml, quickly uncouples mitochondria in PE culture cells in medium 199. The cell ultrastructure undergoes reversible changes (especially that of mitochondria): maximum changes occur 2 hours after the start of the treatment; in 8 hours they become less pronounced. The adaptation of cells does not involve the ionophore inactivation in the medium. 10 micrograms/ml of A23187 induces gradual but irreversible alterations. Microtubules in PE cells are not destroyed when incubated in medium 199 containing 10 micrograms/ml of A23187 and 11 mM Ca2+. The addition of 10 micrograms/ml ionophore to the normal 199 medium (1.26 mM Ca2+) results in the formation of electron dense bodies in the cell center 30 minutes after the start of incubation. These bodies disappear in the course of a subsequent incubation. The number of cells with primary cilia decreases. The percentage of centrioles located perpendicularly to the substrate increases 30 minutes following treatment with 0.1 microgram/ml A23187 in medium 199. 2 hours after the start of treatment with 0.1 microgram/ml ionophore no such changes are detected; an electron dense halo appears around the centriolar cylinders. 8 hours after the start of treatment the structure of the cell center does not differ from the normal one.


PMID: 2473560

Other references

Shah, A.; Osaba, G., 1981: Some new records of powdery mildews from India. Indian phytopathology (pub 1981) 33(2): 320-321

Edwards, G.; Hosking, E.; Smith, S., 1950: Dancing display of the wheatear. A dance display of Oenanthe oenanthe, of which there are only 2 previous reports in the literature, is descr.

Karnath, H.-Otto; Dieterich, M., 2006: Spatial neglect - a vestibular disorder?. The phenomenon of spatial neglect after right brain damage greatly helps our understanding of the normal mechanisms of directing and maintaining spatial attention, of spatial orientation, and the characteristics of neural representation of space....

Rooney, M.; Creurer, I., 1988: Stability of propranolol hydrochloride suspension and solution. American Journal of Hospital Pharmacy 45(3): 530-531

Chik, C.L.; Ho, A.K.; Brown, G.M., 1989: Effect of pinealectomy on the undernutrition-induced suppression of the reproductive axis in rats. Food restriction is known to sensitize the reproductive axis of rats to the inhibitory action of the pineal gland and its hormone, melatonin. The present study investigated the effect of pinealectomy on the undernutrition-related reproductive supp...

Sargent Thomas Edward Hartley, 1945: The organization and work of the B C Department of Mines. Western Miner (Vancouver) 18(8): 54-55

Anonymous, 2012: Novel therapeutic agents for cutaneous T-Celllymphoma. Mycosis fungoides (MF) and Sezary Syndrome (SS) represent the most common subtypes ofprimary Cutaneous T-cell lymphoma (CTCL). Patients with advanced MF and SS have apoor prognosis leading to an interest in the development of new therapies with ta...

Schroeter J.; Brusis T.; Luckhaupt H., 1985: Comparison of measurements of the extent of interaural transmission with regard to the problem of noise exposures differing between 2 sides at the work place. This paper was initiated by the publication of Pfeiffer and Maue which dealt with asymmetric noise exposures at working places. The question of whether one could use the concha position for a microphone to measure the interaural level differences...

Costantini, S.; Rossi, M.; Colonna, G.; Facchiano, A.M., 2005: Modelling of HLA-DQ2 and its interaction with gluten peptides to explain molecular recognition in celiac disease. Celiac disease (CD) is sustained by abnormal intestinal mucosal T-cell response to gluten and it is strongly associated with HLA class 11 molecules encoded byDQA1 *0501/DQB1 *02 (DQ2) or DQA1 *03/DQB 1 *0302 (DQ8). The in vitro stimulatory activit...

Barnes, P.J.; Celli, B.R., 2009: Systemic manifestations and comorbidities of COPD. Increasing evidence indicates that chronic obstructive pulmonary disease (COPD) is a complex disease involving more than airflow obstruction. Airflow obstruction has profound effects on cardiac function and gas exchange with systemic consequences....