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Reassortants of influenza B viruses for use in vaccines: an evaluation


, : Reassortants of influenza B viruses for use in vaccines: an evaluation. Archives of Virology 83(3-4): 169-179

Three slightly different procedures for the preparation of influenza B virus reassortants from B/Lee/40 and B/Lyon/79, and B/Johannesburg/58 and B/Hong Kong/82 parental viruses are described. Following cloning procedures in eggs or allantois-on-shell cultures in the presence of antisera to B/Lee or B/Johannesburg, twenty-eight putative reassortants were obtained. Using SDS-PAGE to determine the migration rates of virion polypeptides, eighteen isolates from mixed infections of B/Lee with B/Lyon, and three isolates from mixed infections of B/Johannesburg with B/Hong Kong, were found to be reassortants. All reassortants possessed surface proteins derived from either B/Lyon or B/Hong Kong and one to three internal polypeptides from either B/Lee or B/Johannesburg. None of the reassortants showed a growth capacity in embryonated eggs as high as that of their B/Lee or B/Johannesburg parent viruses. The procedures used, and the influenza B reassortants thus generated, would appear to offer no major advantages over the existing methods and influenza B strains at present employed for the preparation of inactivated influenza virus vaccines.

US$29.90

PMID: 3155942


Other references

Alekseeva L.A., 1968: Effect of aldactone a renal acting on urinary excretion of electrolytes in patients with myo cardial infarction human hypothiazide renal acting novurit renal acting. Terapevticheskii Arkhiv 40(1): 51-56

Sperk G.; Marksteiner J.; Meyer D.K., 1988: Differential responses of somatostatin npy cck 8 and vip in kainic acid induced epilepsy in the rat. Society for Neuroscience Abstracts 14(1): 6

Mrsulja, B.B.; Mrsulja, B.J.; Spatz, M.; Ito, U.; Walker, J.T.Jr ; Klatzo, I., 1976: Experimental cerebral ischemia in mongolian gerbils part 4 behavior of biogenic amines. Behavior of biogenic amines was studied in the brains of Mongolian gerbils subjected to unilateral occlusion of the common carotid artery. Assays on the hemispheres ipsilateral to occlusion revealed in symptom-positive animals a progressive decrea...

Meijer, L.; Ostvold, A.C.; Walass, S.I.; Lund, T.; Laland, S.G., 1991: High-mobility-group proteins P1, I and Y as substrates of the M-phase-specific p34cdc2/cyclincdc13 kinase. All dividing cells entering the M phase of the cell cycle undergo the transient activation of an M-phase-specific histone H1 kinase which was recently shown to be constituted of at least two subunits, p34cdc2 and cyclincdc13. The DNA-binding high-...

Horky, M.; Kankova, K.; Kolar, F.; Vojtesek, B., 1999: Increased expression of VEGF is associated with diminished apoptosis and maintained proliferation of endothelial cells in myocardium during the early postnatal development. Journal of Molecular & Cellular Cardiology 31(6): A103, June

Myers, G.S.Roberts, T.R., 1968: Note on dentition of Creagrudite maxillaris, a characid fish from the Upper Orinoco-Upper Rio Negro system. Stanford Ichthyological Bulletin, 8: 248-249

McLeod, B.C.; Viernes, A.L.; Sassetti, R.J., 1983: Serum-free light chain analysis by crossed immunoelectrophoresis: correlation with plasmapheresis in light chain disease nephropathy. The technique of crossed immunoelectrophoresis (X-IEP) has been used to quantify free monoclonal light chains (LC) directly in the serum of patients with light chain disease, without preliminary gel or membrane filtration of serum to separate whol...

Grad, K.; Dozet, S.; Petrica, R.; Rijavec, L., 1997: Pseudosocka beds with coal in borehole Tdp-1/84 Trobni Dol (eastern Sava folds, Slovenia). Geologija (Ljubljana). 1996( ); 39: 97-118

Curculio, 1951: Rodent control. The infestation of food warehouses by rats is reviewed and examples of infestations in Puglia, Italy and in Texas pointed out.

Nishiuchi, Y., 1990: Pesticide toxicity to the tadpole. The toxicities to the tadpoles of Bufo bufo japonicus of 145 pesticides were tested. The formulations of the pesticides affected the toxicity, with the order of toxicity to the tadpoles as follows: emulsifiable concentrate >= technical grade &g...