geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Separation of five isoenzymes of serum lactate dehydrogenase by discontinuous gradient elution from a miniature ion-exchange column


, : Separation of five isoenzymes of serum lactate dehydrogenase by discontinuous gradient elution from a miniature ion-exchange column. Clinical Chemistry 25(8): 1453-1458

We describe a simple, fast chromatographic technique for quantitatively separating the five isoenzymes of lactate dehydrogenase (LD; EC 1.1.1.27) in serum. A 250-microL serum sample is applied to a 6.0 x 0.7 cm column of QAE-Sephadex A-50 and eluted stepwise with five different buffers. The isoenzyme fractions, assayed by the method of Wroblewski and LaDue [Proc. Soc. Exp. Biol, Med. 90, 210 (1955)], are stable at room temperature for 24 h. For all five isoenzymes the average within-day coefficient of variation is 4.3%; the day-to-day CV for 20 days is 6.3%. Of some common potentially interfering substances tested, only sodium fluoride (238 mmol/L) was found to do so, by slowing the elution from the column and making the fractions turbid. The expected range in international (IUB) united and percent for each isoenzyme was determined from data on 73 men and 70 women. From these data we calculate, by a percentile estimate of a nongaussian distribution, a normal range of LD-1/LD-2 ratio of 0.55--0.87 for men and 0.52--0.91 for women. The LD isoenzyme patterns in both normal and above-normal samples of 147 sera, as evaluated by the present column method and by electrophoresis, correlated well.

US$29.90

PMID: 455684


Other references

Anonymous, 2002: Opinion on ethical aspects of patenting inventions involving human stem cells. The European Group on Ethics in Science and New Technologies to the European Commission. 7 May 2002. Revista de Derecho Y Genoma Humano 16): 266-271

Ferrucci, L.; Guralnik, J.M., 2003: Inflammation, hormones, and body composition at a crossroad. American Journal of Medicine 115(6): 501-502

Kruse K.; Kracht U.; Wohlfart K.; Kruse U., 1988: Intact serum parathormone pth 1 84 a suitable parameter for the diagnosis of abnormalities of calcium metabolism. Intact parathormone (PTH 1-84) was measured with a new immunoradiometric method in serum from 83 children and adults with various abnormalities of calcium metabolism. The results were compared with those of an assay of midregional PTH fraction (44...

Woods, 1967: The sick person; center of our interest. Bulletin des Infirmieres Catholiques du Canada 34(1): 22-23

Rossi A.M., 1977: Cell stages and caffeine influence on x radiation induced mutability in wild type and a mutator strain of schizosaccharomyces pombe. Atti Associazione Genetica Italiana: 56

Krupski, A.; Sears, J.M.; Joesch, J.M.; Estee, S.; He, L.; Dunn, C.; Huber, A.; Roy-Byrne, P.; Ries, R., 2010: Impact of brief interventions and brief treatment on admissions to chemical dependency treatment. This study examined two issues. One, whether individuals with possible substance use disorders were more likely to be admitted to specialized chemical dependency (CD) treatment after receiving a brief intervention (BI) - either alone or in combina...

Abdulkadir J., 1985: Utilization of traditional treatment among ethiopian diabetics. The incidence of utilization of traditional treatment of diabetes among 253 consecutive Ethiopian diabetics, including all age-groups above 13 yr was studied and a positive history was obtained in 65 (25.7%); in 32.3% of 99 insulin-treated, and in...

Miltenburger, H.G.; Bauer, A., 1975: Simultaneous effect of 5-bromodeoxyuridine and cysteamine on x-irradiated diploid cells of Chinese hamster, Cricetulus griseus, in vitro. Cells of the Chinese hamster line B 14 FAF 28 were treated with BUdR and irradiated with X-rays under the presence of cysteamine. The dose-dependent inactivation rates could be determined rather precisely by marking single cells. Daily cell counts...

Schmuker, M.; Yamagata, N.; Nawrot, M.Paul.; Menzel, R., 2012: Parallel representation of stimulus identity and intensity in a dual pathway model inspired by the olfactory system of the honeybee. The honeybee Apis mellifera has a remarkable ability to detect and locate food sources during foraging, and to associate odor cues with food rewards. In the honeybee's olfactory system, sensory input is first processed in the antennal lobe (A...

Uchiyama, R.C.; Osborn, T.G.; Moore, T.L., 1989: Antibodies to iris and retina detected in sera from patients with juvenile rheumatoid arthritis with iridocyclitis by indirect immunofluorescence studies on human eye tissue. Sera from 12 patients with juvenile rheumatoid arthritis (JRA) with active iridocyclitis were incubated with frozen, sectioned, whole human eye tissue. Antibodies were detected using fluorescein conjugated goat F(ab')2 antibody to human IgG b...