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Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5) methyltransferase


, : Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5) methyltransferase. Biochemical and Biophysical Research Communications 145(1): 146-152

M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA (cytosine-5) methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstituted strand.

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PMID: 3036116


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