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Stability, targeting, and biodistribution of scandium-46- and gallium-67-labeled monoclonal antibody in erythroleukemic mice

, : Stability, targeting, and biodistribution of scandium-46- and gallium-67-labeled monoclonal antibody in erythroleukemic mice. Cancer Research 45(5): 2154-2158

Using a monoclonal antibody specific for the Mr 70,000 glycoprotein of Rauscher erythroleukemia virus, we have determined the optimal conditions for conjugation with the cyclic anhydride of diethylene triamine pentaacetic acid and subsequent labeling with 46Sc. The conjugates were shown to retain their specificity and activity in vitro and to target specifically to virus-infected spleen cells in vivo. The stability of the 46Sc:diethylene triamine pentaacetic acid-antibody conjugates in vivo was studied using immunoaffinity chromatography; 25% of the isotope bound to transferrin, and 75% remained bound to the antibody conjugates. These results are discussed with respect to the potential for labeling antibodies with 47Sc for use in imaging and therapy. Studies with 111In-labeled antibody were used for comparison. Labeling with 67Ga was also performed; these labeled conjugates showed adventitious binding of isotope to the antibody and lack of stability of diethylene triamine pentaacetic acid-chelated gallium. Free EDTA was shown to stably incorporate 67Ga.


PMID: 3857116

Other references

Claus Klamp F.L.; Mayoral E., 1992: Upper Miocene Skolithos ichnofacies on the southern edge of the Guadalquivir Basin, Arcos de la Frontera, Cadiz. Geogaceta 12: 102-103

Walker, N., 1997: Metabolism of chloropehnols by Rhodotorula glutinis. Soil biology and biochemistry: 3, 5 (5) 525-530

Alarcón, G.S.; Rodríguez, J.L.; Benavides, G.; Brooks, K.; Kurusz, H.; Reveille, J.D., 2000: Systemic lupus erythematosus in three ethnic groups. V. Acculturation, health-related attitudes and behaviors, and disease activity in Hispanic patients from the LUMINA cohort. LUMINA Study Group. Lupus in Minority Populations, Nature versus Nurture. To assess the relationship between acculturation and clinical, socioeconomic-demographic, and behavioral/psychosocial features in Hispanic patients with systemic lupus erythematosus (SLE) from the LUMINA (Lupus in Minority Populations, Nature vers...

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Popov, D., 1977: Mechanization for the growing and harvesting of some vegetables in France. Selskostopanska tekhnika: 4 (5) 89-93

Wadud, S.; Kosar, S.; Fazal, H.A.a; Abid, H., 1992: Bacteriological status of drinking water in rural areas of Peshawar. A study was carried out on the occurance and distribution of different types of bacteria present in the drinking water of 12 villages near Peshawar from different sources i.e. well, tube-well, canal and river. 120 samples were analysed and the dif...

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Mamluk, O.F.;, M.; Makki, M.A., 1990: Current status of wheat diseases in Syria. During 4 growing seasons (1984-85, 1987-88), 118 wheat fields representing all major wheat areas in Syria were examined for disease incidence and severity. The most widely recorded diseases were common bunt (Tilletia foetida [T. laevis]), Septoria...

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