+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The effect of metoclopramide and dopamine on mineralocorticoid secretion--in vivo and in vitro studies

, : The effect of metoclopramide and dopamine on mineralocorticoid secretion--in vivo and in vitro studies. Nihon Naibunpi Gakkai Zasshi 62(3): 197-207

There is considerable information suggesting that dopamine is a physiological regulator of aldosterone secretion. Metoclopramide, a specific dopamine antagonist, elicits a rapid rise in plasma aldosterone independent of the known aldosterone-regulating factors. However, the mechanism and the site of action of metoclopramide, whether adrenal or extra-adrenal, in stimulation of aldosterone production remain to be defined. The present studies were designed to investigate the mechanism of dopaminergic control of corticosteroid secretion and to determine at which step in the aldosterone biosynthetic pathway metoclopramide and dopamine exert their effect. Plasma concentrations of progesterone, 11-deoxycorticosterone (DOC), and cortisol were not altered by a bolus intravenous administration of 10 mg metoclopramide in 8 healthy male volunteers. Metoclopramide increased plasma aldosterone from 6.9 +/- 2.8 (Mean +/- 2SD) ng/100 ml to a maximum level of 18.2 +/- 4.7 ng/100 ml, 18-hydroxycorticosterone (18-OHB) from 12.6 +/- 6.5 ng/100 ml to a maximum of 41.3 +/- 7.3 ng/100 ml and corticosterone from 0.36 +/- 0.09 microgram/100 ml to a maximum of 0.85 +/- 0.22 microgram/100 ml. The aldosterone, 18-OHB and corticosterone responses displayed a parallel time course, with a significant response of each occurring within 5 minutes after metoclopramide administration. These data suggest that metoclopramide may modulate the activities of 18-hydroxylase and 11 beta-hydroxylase. Studies in vitro revealed that metoclopramide (10(-8)-10(-4) M had little effect on basal production of aldosterone, 18-OHB and corticosterone from human adrenal slices. Dopamine (10(-4) M) did not alter the basal secretion of aldosterone, 18-OHB and corticosterone, but suppressed the secretion of these 3 mineralocorticoids by ACTH, which were diminished by addition of 10(-4) M metoclopramide. There was a concentration-dependent inhibitory effect of dopamine on conversion of corticosterone to 18-OHB and DOC to corticosterone in vitro using bovine adrenal mitochondrial fractions. IC50 of dopamine inhibiting 18-hydroxylation and 11 beta-hydroxylation were 7.5 X 10(-7) M and 9.5 X 10(-4) M, respectively. It appears that physiological concentration of dopamine can modulate the activity of 18-hydroxylase enzyme. In summary, it can be concluded that the in vivo and in vitro studies are compatible with a view that dopamine has a physiological role in the regulation of aldosterone by modulating the activity of 18-hydroxylase enzyme.


PMID: 3709883

Other references

Din, L., B.; Latiff, A.; Zakaria, Z.; Elix, J.A., 2002: Chemical constituents of the lichen, Heterodermia flabellata and H. leucomelos (Physiaceae) in Peninsular Malaysia. The chemical constituents of the lichen Heterodermia flabellata and H. leucomelos (Physiaceae) collected from Gunung Jerai, Kedah and Cameron Highlands, respectively, are described. The latter is a new record for Peninsular Malaysia.

Carothers H.P., 1965: Engineered foundations in expansive clay. Pages 302-323 1965

Kramer, A., 1966: Sichtmarkierung bei Gemsen und andern einheimischen Huftieren. Revue Suisse de Zoologie, 73: 400-407

Turner, D.A., 1982: Reflux of radiopharmaceutical into the inferior vena cava during first-transit studies of the heart. Journal of Nuclear Medicine 23(12): 1141-1141

Nagy, A.; Rajki, K.; Horvath, L.; Csanyi, V., 1978: Investigation on carp cyprinus carpio gynogenesis. A reliable method for gynogenetic mass-production was developed in carp (C. carpio L.). The effect of different cold shock treatments applied after fertilization were examined. The yield of viable diploid gynogenetic animals could be significantly...

Strel' kova, M.V.; Alekseev, A.N.; Passova, O.M., 1981: Changes in the virulence of strains of the causative agent of zoonotic cutaneous leishmaniasis after passaging in Phlebotomus papatasi. Female P. papatasi were fed with dosed cloned culture of Leishmania tropica major of high or low virulence and with mixed low:high virulence clones at ratios of 1:1 and 1:50. The virulence (to hamsters and mice) of the passaged strain (longest per...

Dominguez, A.; Salleras, L.; Cardenosa, N.; Ciruela, P.; Carmona, G.; Martinez, A.; Torner, N.; Fuentes, M., 2002: The epidemiology of invasive Streptococcus pneumoniae disease in Catalonia (Spain). A hospital-based study. The aim of this study was to investigate the incidence of invasive pneumococcal disease (IPD) in Catalonia. A hospital-based incidence study of the period 1997-1999 was carried out by reviewing the isolations of Streptococcus pneumoniae obtained f...

Cardinaels, E.P.M.; Daamen, Mëlle.A.M.J.; Bekers, O.; ten Kate, J.; Niens, M.; van Suijlen, J.D.E.; van Dieijen-Visser, M.P.; Brunner-L.R.cca, H-Peter.; Schols, J.M.G.A.; Mingels, A.M.A., 2016: Clinical Interpretation of Elevated Concentrations of Cardiac Troponin T, but Not Troponin I, in Nursing Home Residents. Cardiac troponins T (cTnT) and I (cTnI) are the preferred biomarkers to detect myocardial damage. The present study explores the value of measuring cardiac troponins (cTn) in nursing home residents, by investigating its relation to heart failure a...

Tall, M., 1982: The apartment way to grow cacti and succulents from seeds. Cactus and succulent journal 54(4): 169

Strzelecka-Kiliszek, A.; Buszewska, M.E.; Podszywalow-Bartnicka, P.; Pikula, S.; Otulak, K.; Buchet, R.; Bandorowicz-Pikula, J., 2007: Calcium- and pH-dependent localization of annexin A6 isoforms in Balb/3T3 fibroblasts reflecting their potential participation in vesicular transport. Annexin A6 (AnxA6), calcium- and membrane-binding protein, is involved in membrane dynamics. It exists in the cell in two isoforms, AnxA6-1 and AnxA6-2, varying only by the VAAEIL sequence. In most cells, AnxA6-1 predominates. A limited number of...