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The endogenous nuclease sensitivity of repaired DNA in human fibroblasts

, : The endogenous nuclease sensitivity of repaired DNA in human fibroblasts. Mutation Research 184(2): 169-178

The limited DNA excision repair that occurs in the chromatin of UV-irradiated growth arrested cells isolated from a xeroderma pigmentosum (XP) complementation group C patient is clustered in localized regions. The repaired DNA was found to be more sensitive to nicking by endogenous nucleases than the bulk of the DNA. The extra-sensitivity does not change with increasing amounts of DNA damage or repair activity in the locally-repaired regions and is retained through a 24-h chase period. We suggest that these results are due to the occurrence of DNA repair limited to pre-existing, non-transient chromatin fractions that contain actively transcribed DNA. A similar extra-sensitivity of repaired DNA was not detected in cells of normal or XP complementation group A strains that exhibit either normal or limited repair located randomly throughout their genomes. The association between endogenous nuclease sensitivity and clustered repair probably defines a normal excision repair pathway that is specific for selected chromatin domains. The repair defect in XP-C strains may be one in pathways targeted for other endogenous nuclease-resistant domains.


PMID: 3627144

Other references

Larbier M.; Blum J C., 1975: The effects of methionine when supplemented in the diet or injected into eggs on embryo and post natal development of chicks. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles 280(18): 2133-2136

Riis, P., 1991: The Nordic concept of 'faellesskab'. The complex of cultural, political and societal affiliations, both in a historic and a contemporary perspective, is expressed by a special term in the Nordic languages, 'faellesskab', often with the addition of 'folkelig', as &...

Mortensen, E.M.; Metersky, M.L., 2012: Long-term mortality after pneumonia. Although pneumonia is currently considered the eighth leading cause of death in the United States this estimate includes only short-term mortality. Evidence suggests that pneumonia may have significant longer-term effects and that hospitalization...

Do, Q.Huu.; Zeng, C.; Zhang, C.; Wang, B.; Zheng, J., 2011: Supercritical fluid deposition of vanadium oxide on multi-walled carbon nanotube buckypaper for supercapacitor electrode application. Composite electrodes were fabricated for supercapacitor applications by depositing vanadium oxide onto multi-walled carbon nanotube (MWCNT) buckypaper using supercritical fluid deposition (SFD). The deposited thin vanadium oxide layer showed amorp...

Onai, T.; FitzGerald, M.G.; Arakawa, S.; Gocayne, J.D.; Urquhart, D.A.; Hall, L.M.; Fraser, C.M.; McCombie, W.R.; Venter, J.C., 1989: Cloning, sequence analysis and chromosome localization of a Drosophila muscarinic acetylcholine receptor. Two cDNA clones (3.7 kb and 4.8 kb) encoding a Drosophila muscarinic acetylcholine receptor were isolated from a Drosophila head cDNA library and characterized by automated DNA sequence analysis. The Drosophila muscarinic receptor contains 788 ami...

Kopelman, P., 2007: Health risks associated with overweight and obesity. Discussions on the role of obesity and overweight in causing or exacerbating health problems, independently and in association with other diseases, are presented. The specific topics discussed include: risks and diseases associated with increased...

Ozer, Törün.; Başaran, Güvenç.; Kama, J.Devecioğlu., 2010: Surface roughness of the restored enamel after orthodontic treatment. After fixed appliance treatment, one concern is to restore the enamel surface as closely to its original state as possible. A variety of cleanup processes are available, but all are time-consuming and carry some risk of enamel damage. The purpose...

Foo, K.Y.; Hameed, B.H., 2010: Value-added utilization of oil palm ash: a superior recycling of the industrial agricultural waste. Concern about environmental protection has increased over the years from a global viewpoint. To date, the infiltration of oil palm ash into the groundwater tables and aquifer systems which poses a potential risk and significant hazards towards the...

Hurley, J., 1981: A history of the occurrence of Lymnaea glabra Gastropoda Pulmonata in Ireland. Irish Naturalists' Journal, 207: 284-287

Grassi, B., 1885: Intorno ad alcuni protozoi parassati delle Termiti. T;: 235-240