+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The kappa-deleting element. Germline and rearranged, duplicated and dispersed forms

, : The kappa-deleting element. Germline and rearranged, duplicated and dispersed forms. Journal of Experimental Medicine 167(2): 488-501

Human light chain genes are used in a kappa before lambda order. Accompanying this hierarchy is the rearrangement of a kappa-deleting element (Kde) which eliminates the kappa locus before lambda gene rearrangement. In approximately 60% of rearrangements the Kde recombines at a conserved heptamer within the J kappa-C kappa intron. We demonstrated that aberrant V/J rearrangements possessing apparent "N" nucleotides existed 5' to the J kappa-Kde rearrangements. This suggests that the Kde may selectively eliminate nonfunctional V/J alleles. A kappa-producing cell that displayed the unusual finding of lambda gene rearrangement demonstrated a rearranged Kde. This rearrangement was a V kappa/Kde recombination and the heptamer-11 bp spacer-nonamer flanking the V kappa is the target site of the Kde 40% of the time. The mouse possesses a counterpart to the Kde (recombining sequence [RS]) and the highly conserved regions surround the heptamer-spacer-nonamer signals. No complete protein product was predicted from the germline Kde near its break-point and no consistent fusion product was predicted from either the V/Kde or V/J-Kde rearrangements. A distal portion of the Kde is duplicated and is present at 2q11 as well as 2p11. The evolutionary conservation of the kappa-elimination event, the duplication and maintenance of the Kde indicates that it has a function. A portion of the Kde may still prove to encode a trans-acting factor that directly affects lambda rearrangement. A certain role for the Kde is its site-specific rearrangement, which destroys ineffective kappa genes and sets the stage for lambda gene utilization.


PMID: 3126251

Other references

D.C.sare, M.; Pratesi, G.; Perego, P.; Carenini, N.; Tinelli, S.; Merlini, L.; Penco, S.; Pisano, C.; Bucci, F.; Vesci, L.; Pace, S.; Capocasa, F.; Carminati, P.; Zunino, F., 2001: Potent antitumor activity and improved pharmacological profile of ST1481, a novel 7-substituted camptothecin. Relevant drawbacks of the molecular structure and mechanism of the action of camptothecins are the instability of the E ring lactone and the reversibility of drug-target interaction. Such features are expected to limit the clinical efficacy of con...

Hibi, K.; Mizutani, M.; Imazawa, M.; Nakamura, T.; Nonoyama, M.; Shibata, H., 2008: Mucinous cystadenoma of the appendix associated with muscular and neuromatous hyperplasia: report of a case. Mucinous cystadenoma of the appendix is a type of appendiceal mucocele and a rather rare condition usually found incidentally in the course of other abdominal surgery. A previous evaluation of 73 appendiceal mucoceles showed that this disease was...

Turner, N.C.; Dolan, J.S.; Grimsditch, D.; Lamb, J.; Worby, A.; Murray, K.J.; Coates, W.J.; Warrington, B.H., 1994: Pulmonary effects of type V cyclic GMP specific phosphodiesterase inhibition in the anaesthetized guinea-pig. 1. We have investigated the bronchodilator potential of type V phosphodiesterase (PDE V) inhibitors in anaesthetized ventilated guinea-pigs using the potent and selective PDE V inhibitor, SK&F 96231. We have compared its activity to that of sa...

Goebel, Dr., 1932: Value of cheap protein mixtures in pig feeding. Experiments conducted at 5 different centres with 112 pigs are described. At all centres " Sass " food mixture (Sassfuttermischung) was tested against mixtures of other protein foods such as whale meal, soya meal, earth-nut meal, fish me...

Pershing L.K.; Corlett J.; Jorgensen C.; Krueger G.G.; Shah V.P.; Wright E.D., 1992: Disparity in ketoconazole activity in vitro versus tinea pedis subjects following oral and topical administration. Journal of Investigative Dermatology 98(4): 651

Fedio, P.; August, A.; Sato, S.; Van Buren, J.; Kufta, C., 1993: Left and right brain stimulation and reversed laterality of language and spatial functions Fixed specialization versus transfer of functions. Epilepsia 34(SUPPL 6): 70

van der Ven, E.; Bourque, Fçois.; Joober, R.; Selten, J-Paul.; Malla, A.K., 2013: Comparing the clinical presentation of first-episode psychosis across different migrant and ethnic minority groups in Montreal, Quebec. To explore differences in severity and nature of symptoms of first-episode psychosis (FEP) according to ethnic group and migrant status. We administered rating scales to assess positive and negative symptoms, as well as general psychopathology, to...

Armstrong, M.D.; Stave, U., 1973: Emergency care for acute poisoning with phosphororganic compounds. Metabolism: Clinical and Experimental 22(4): 571-578

Ronnholm, Aar, 1972: Reducing evaporation plant pollution and its treatment. Paperi ja puu: 54 (11) 715-716

Helle, S.C.J.; Kanfer, G.; Kolar, K.; Lang, A.; Michel, Aès.H.; Kornmann, Bît., 2013: Organization and function of membrane contact sites. Membrane-bound organelles are a wonderful evolutionary acquisition of the eukaryotic cell, allowing the segregation of sometimes incompatible biochemical reactions into specific compartments with tailored microenvironments. On the flip side, these...