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The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel

, : The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. Cell 8(3): 333-347

We have examined the role played by various histones in the organization of the DNA of the nucleosome, using staphylococcal nuclease as a probe of DNA conformation. When this enzyme attacks chromatin, a series of fragments evenly spaced at 10 base pair intervals is generated, reflecting the histone-DNA interactions within the nucleosome structure. To determine what contribution the various histones make to DNA organization, we have studied the staphylococcal nuclease digestion patterns of complexes of DNA with purified histones. Virtually all possible combinations of homogeneous histones were reconstituted onto DNA. Exhaustive digestion of a complex containing the four histones H2A, H2B,H3, and H4 yields a DNA fragment pattern very similar to that of whole chromatin. The only other combinations of histones capable of inducing chromatin-like DNA organization are H2A/H2B/H4 and those mixtures containing both H3 and H4. From an examination of the kinetics of digestion of H3/H4 reconstitutes, we conclude that although the other histones have a role in DNA organization within the nucleosome, the arginine-rich histone pair, H3/H4, can organize DNA segments the length of the nucleosome core in the absence of all other histones.


PMID: 986252

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Straznickas, K.A.; McNiel, D.E.; Binder, R.L., 1993: Violence toward family caregivers by mentally ill relatives. Hospital & Community Psychiatry 44(4): 385-387

Meyer, P.J.; Allen, S.K., 1983: Spontaneous iliac artery-inferior vena caval fistula: rare complication of ruptured abdominal aneurysm. Journal of the American Osteopathic Association 83(1): 52-55

Birndorf, N.I.; Lopas, H., 1969: Evaluation of stroma free hemoglobin solution: effects on renal function in cynomolgus monkeys. Rep No 838. Report. Army Medical Research Laboratory: 1-13

Cavallini, B.; Ronconi, G.; Baiguini, E., 1976: Corrected transposition of the great vessels. A clinical review of 21 cases. Clinical and instrumental features of 21 cases of congenitally corrected transposition of the great vessels are reviewed. We confirm the rarity of the isolated form of the disease and its frequent association with ventricular septal defect and pul...

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Mills D.C.B., 1974: Factors influencing the adenylate cyclase system in human blood platelets. Sherry, Sol And Alexander Scriabine (Ed ) Platelets And Thrombosis Symposium Philadelphia, Pa , U S A , Nov 20-21, 1972 Xvi+332p Illus University Park Press: Baltimore, Md , U S A ; London, England 45-67

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Hillman B.; Morris T.J.; Kellen W.R.; Hoffman D.; Schlegel D.E., 1982: An invertebrate calicivirus like virus evidence for partial virion disintegration in host excreta. A virus with morphological and physicochemical properties similar to those of the vertebrate caliciviruses was isolated from navel orangeworms, Amyelois transitella (Walker). Infected larvae contained 2 types of virus particles: a 185S, 38 nm cupp...

Vaccaro, A.; Sivieri, A., 1969: Frequency of infection by Stephanostomum (Looss, 1899) sp. larvae in Mullus barbatus (L.) and Mullus surmuletus (L,), caught in the upper, middle and lower Tyrrhenian Sea. Examination of 500 Mullus barbatus and 500 M. surmuletus from the Tyrrhenian Sea showed them all to be infected with Stephanostomum sp. larvae which are briefly described.

Heyck, H.; Hopker, W., 1952: Brain changes in the rat by ultrasound. Histological changes of the brain after exposures to various frequencies and of various duration were descr. In the ganglionic cells shrinkage occurred,in the later stages swelling and pyknosis occurred. Higher frequencies cause more damage. This...