geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The phosphorylation of the primary gene products of alpha-crystallin


, : The phosphorylation of the primary gene products of alpha-crystallin. Journal of Biological Chemistry 262(4): 1438-1441

The alpha-crystallin primary gene product A2 and its post-translational modified counterpart A1 were isolated from calf lens cortex. The amino acid compositions determined from both chains were almost identical and in excellent agreement with that calculated from the reported sequence of A2. Chemical analysis of phosphate revealed 1 mol/mol of A1 and was negative in A2. Phosphoamino acid analysis demonstrated the presence of phosphoserine only in A1. Chymotryptic peptide maps of A2 and A1 resolved approximately 50 peptides and were strikingly similar. An apparent change in the relative mobility of one peptide was the only difference observed between A1 and A2. Phosphate analysis of this peptide obtained from A1 and A2 was positive only in the peptide from A1. Identical amino acid composition and the sequence Arg-Leu-Pro-Ser-Asn-Val-Asp-Gln-Ser-Ala-Leu was found for the peptide isolated from both chains, corresponding to residues 119 to 129 in the reported sequence of A2. These results indicate that the post-translational modification of A2 to A1 is the result of a phosphorylation reaction rather than a spontaneous nonenzymatic deamidation as previously suggested.

US$29.90

PMID: 3805033


Other references

Anonymous, .: Pasture development - Carimagua. In trials begun in 1980, 4 mixtures of Brachiaria humidicola or Andropogon gayanus with Desmodium ovalifolium or Pueraria phaseoloides were sown in strips 0.5, 2.5 or 5.0 m wide representing 20% of the total area of savanna with an annual increase...

Campbell, R., 1992: The neuropsychology of lipreading. Lipreading presents a unique glimpse of the intersection of sensory processes with modular, cognitive ones. It presents speech to the eye in an automatic and natural way, whether performed silently or in conjunction with heard speech. It therefore...

Whitehouse, M.E., 1991: To mate or fight? Male-male competition and alternative mating strategies in Argyrodes antipodiana (Theridiidae, Araneae). Argyrodes antipodiana is a kleptoparasitic spider that builds its own web around the webs of other, larger host species. Males are more prone to have contests on webs of conspecific females than on webs of conspecific males. Males are also more li...

Clark, S., 1990: Presentation European-style. Milk product packaging innovations in France, Austria, Denmark, Italy, German Federal Republic and Japan are described, and compared with UK milk product packaging.

Hinder, R.A.; Napoliello, D.A.; Klingler, P.J., 1997: Cutting edge management of achalasia. American Journal of Gastroenterology 92(7): 1078-1079

Singh, S.-Kumar; Kumar, A.; Singh, A.-Kumar; Singh, J.P.N., 2007: Histology and histochemistry of club cells in the epidermis of a fresh water fish, Clupisoma garua. Club cells are observed in the epidermis of Clupisoma garua in present investigations. These are voluminous and beinucleated. Their secretory contents stain strongly for different classes of proteins. In addition, the club cells of Clupisoma garu...

Malloggi, F.; Andreotti, B.; Clément, E., 2016: Nonlocal effects in sand flows on an inclined plane. The flow of sand on a rough inclined plane is investigated experimentally. We directly show that a jammed layer of grains spontaneously forms below the avalanche. Its properties and its relation with the rheology of the flowing layer of grains are...

Heide, R.M. van der, 1988: The rise and fall of malaria in the Netherlands. The history of indigenous malaria in the Netherlands since 1600 is discussed with reference to the presence of the parasite and the intermediate host, the brackish water mosquito Anopheles maculipennis atroparvus [A. atroparvus], the use of chemot...

Brito Neves B.B.; Winge M.; Carneiro M.A., 1996: Orogenesis preceeding and taphrogenesis succeeding Rodinia in South America. Boletim IG-USP, Serie Cientifica 27: 1-40

Chakraborty, B.; Das, D.; Kondev, J., 2004: Topological jamming and the glass transition in a frustrated system. The relationship between extended structures, glassy dynamics and an underlying critical point is examined in the context of a lattice model of fluctuating lines. Monte Carlo simulations are used to construct an effective, coarse-grained dynamics...