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Thermal chromatography of lysine-specific transfer ribonucleic acid from Escherichia coli B


, : Thermal chromatography of lysine-specific transfer ribonucleic acid from Escherichia coli B. Journal of Chromatography 103(2): 355-363

Two Escherichia coli B tRNALys isoacceptors have been separated by thermal chromatography on hydroxyapatite demonstrating the potential of this approach in the fractionation of single-stranded polynucleotides. Elution temperatures were reproducible and depended primarily on the cation present and its concentration. Cesium phosphate was a more effective eluent than sodium phosphate. The integrity of the polynucleotide following thermal chromatography was examined by ultracentrifugation in a 60% dimethyl sulfoxide solvent, electrophoresis on polyacrylamide gels containing urea and oligonucleotide mapping. Adsorption to hydroxyapatite did not noticeably increase phosphodiester bond breakage compared with that in solution. Minimal, if any, rupture of covalent linkages was detected under the conditions of preparative separation of the isoacceptors. Several observations suggest that only one tRNALys was transcribed by the E. coli B cell.

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PMID: 1090634


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Miller, R.R.; Letts, R.L.; Potts, W.J.; McKenna, M.J., 1980: Improved methodology for generating controlled test atmospheres. Improved methodology has been developed for generating controlled test atmospheres for inhalation toxicology studies. Vaporization of volatile liquids is accomplished in a 28 mm (outer diameter) glass J-tube in conjunction with a compressed air fl...

Andrew John A.; Edwards Duncan M.; Graf Robert J.; Wold Richard J., 1991: Empirical observations relating near-surface magnetic anomalies to high-frequency seismic data and Landsat data in eastern Sheridan County, Montana. Our empirical synergistic correlations of aeromagnetic and seismic data and a Landsat lineament interpretation revealed lineations on the magnetic map that have expression on seismic sections. We observed a conjugate set of northwest-southeast and...

Rogov, V.S., 1968: A case of systemic ossifying periostosis (Pierre Marie-Bamberger disease). Voprosy Okhrany Materinstva i Detstva 13(1): 85-86

Naumov A.I.; Beburishvili A.G., 1968: Some clinical and electro cardiographic data on experimental air embolism. Sbornik Nauchnykh Rabot Volgogradskogo Meditsinskogo Instituta 21(2): 202-203

Brady J., 1968: Control of the circadian rhythm of activity in the cockroach iii a possible role of the blood electrolytes periplaneta americana sodium potassium. Journal of Experimental Biology 49(1): 39-47

Allen, H., 1981: Ward sister training. Nursing Times 77(2): 53-53

Stofman, G.M.; Neavin, T.S.; Ramineni, P.M.; Alford, A., 2006: Better sex from the knife? An intimate look at the effects of cosmetic surgery on sexual practices. The positive effects of aesthetic plastic surgery with respect to body image and self-esteem have been reported in the literature. However, the possible effects of aesthetic surgery on the sexual practices and characteristics of patients have been...

Cederlund, R.; Thorén-Jönsson, A-Lisa.; Dahlin, L.B., 2010: Coping strategies in daily occupations 3 months after a severe or major hand injury. Coping strategies used in performing daily occupations 3 months after a severe or major hand injury were explored. A semi-structured interview was performed with 13 participants with hand injuries based on Hand Injury Severity Scoring System and a...

Noirfalise, A.R.isin, M., 1981: Beech forest with wood millet in Belgium (Milio-Fagetum) Fagus.1. Bulletin des recherches agronomiques de Gembloux6(3): 249-256

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