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Thiol-dependent K:Cl transport in sheep red cells: VIII. Activation through metabolically and chemically reversible oxidation by diamide


, : Thiol-dependent K:Cl transport in sheep red cells: VIII. Activation through metabolically and chemically reversible oxidation by diamide. Journal of Membrane Biology 101(2): 179-188

The sulfhydryl (SH) oxidant diamide activated in a concentration-dependent manner ouabain-resistant (OR), Cl-dependent K flux in both low potassium (LK) and high potassium (HK) sheep red cells as determined from the rate of zero-trans K efflux into media with Cl or Cl replaced by NO3 or methane sulfonate (CH3SO3). Diamide did not alter the OR Na efflux into choline Cl. The diamide effect on K efflux appeared after 80% of cellular glutathione (GSH) was oxidized to GSSG, its disulfide. The stimulation of K efflux was completely reversed during metabolic restitution of GSH, a process that depended on the length of exposure to and the concentration of diamide. The action of diamide on both the K:Cl transporter and GSH was also fully reversed by the reducing agent dithiothreitol (DTT). Diamide apparently oxidized the same SH groups alkylated by N-ethylmaleimide (NEM) (Lauf, P.K. 1983. J. Membrane Biol. 73:237-246). Like NEM, diamide activated K:Cl transport several-fold more in LK cells than in HK cells, and the effect on LK cells was partially inhibited by anti-L1, the allo-antibody known to inhibit OR K fluxes.

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PMID: 3367366


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Sommer, O., 1938: On performance and health in German cattle breeding. LIMITATION of the basis of selection to record yields has proved to be irrational, and performance testing is now being extended to include life yield, food utilization, constitution, and fertility. The importance of breeding for soundness and dis...

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Preobrazhenskiĭ, N.A.; Romanenko, D.A., 1978: Surgical treatment in certain forms of peripheral paralysis of the facial nerve (continuation of the review of the foreign literature published in the journal Vestnik Otorinolaringologii 1978 No. 2). Vestnik Otorinolaringologii: 100-101

Claridge G.G.C.; Campbell I.B., 1983: Evidence for high ice levels in the Ross Sea region more than 2 million years ago. New Zealand Antarctic Record: Pages 5-6. 1983.

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Tsuang, M.T., 1975: Sex chromatin anomaly among Chinese psychiatric inpatients compared to schoolchildren. In a study of sex chromatin abnormality among Chinese psychiatric inpatients, buccal smears were examined from a total of 1,253 psychiatric inpatients (639 males and 614 females). One male psychiatric inpatient (1.6/1000) was chromatin positive (p...

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