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Tumorigenic transformation and neoplastic progression of human uroepithelial cells after exposure in vitro to 4-aminobiphenyl or its metabolites


, : Tumorigenic transformation and neoplastic progression of human uroepithelial cells after exposure in vitro to 4-aminobiphenyl or its metabolites. Cancer Research 52(6): 1606-1614

A multistep in vitro/in vivo transformation system was used to test the transforming effect(s) of the human bladder procarcinogen 4-amino-biphenyl (ABP) and two putative proximate carcinogenic metabolites, N-hydroxy-4-aminobiphenyl (N-OH-ABP) and N-hydroxy-4-acetylamino-biphenyl (N-OH-AABP), on a clonally derived nontumorigenic SV40-immortalized human uroepithelial cell line, SV-HUC. SV-HUC were exposed in vitro to concentrations of ABP, N-OH-ABP, or N-OH-AABP that caused a range of cytotoxicity from 5 to 76%. Tumorigenic transformation of SV-HUC, as assessed by the ability of the exposed cells to form carcinomas when inoculated s.c. into athymic nude mice, was achieved after a single exposure to ABP, N-OH-ABP, or N-OH-AABP. In the tumorigenic transformation experiments, 28 of 45 mice representing all 15 carcinogen-exposed observation groups formed carcinomas, whereas none of 9 mice from control groups formed carcinomas (P = 0.001). Neoplastic progression of a low grade regressive squamous cell carcinoma, MC-T11, was also achieved in this system after in vitro exposure to ABP, N-OH-ABP, or N-OH-AABP. In these progression experiments, 11 of 33 mice representing 7 of 12 carcinogen-exposed observation groups formed persistent, high grade nongressing tumors, while only 1 of 19 untreated MC-T11 controls spontaneously progressed on reinoculation (P = 0.022). Forty independent carcinomas generated in athymic nude mice recapitulated diverse cancer phenotypes (including different growth kinetics and histopathological subtypes and grades) represented in clinical bladder cancers. These results demonstrate for the first time the transforming effects of the potent human carcinogen ABP and two of its proximate N-hydroxy metabolites on a prime human target cell type, HUC.

US$29.90

PMID: 1311635


Other references

Paul, W.R.C.; Chelvanayagam, A.V., 1936: Sunnhemp in the Jaffna Peninsula. Aspects of the subject are discussed under the sections systems of cultivation, green manuring, fodder, fibre and economics. The Crotalaria dried leaves, husks and boiled seeds are fed to cattle. On fields where growth has been luxuriant the leafy...

Dijkstra, N.D., 1939: A feeding trial to test the value of artificially dried grass for dairy cows.. A feeding trial was made on 2 groups of 12 milk cows, to determine the amount of concentrates in the ration which could be replaced by artificially dried grass and the limit to which the amount of hay could be reduced. There was no significant dif...

Inamasu, J.; Miyatake, S.; Tomioka, H.; Shirai, T.; Ishiyama, M.; Komagamine, J.; Maeda, N.; Ito, T.; Kase, K.; Kobayashi, K., 2010: Cardiac arrest due to food asphyxiation in adults: resuscitation profiles and outcomes. Food asphyxiation is uncommon but unignorable cause of sudden death in the elderly. Several autopsy studies, which identified those at particular risk, have been conducted on the subject. Resuscitation profiles and outcomes of food asphyxiation vi...

Anonymous, 2012: Novel Mechanism for Sir2 Activation by Isonicotinamide. Sirtuins are an evolutionarily conserved family of NAD+-dependent protein deacetylases that function in the regulation of gene transcription, cellular metabolism, and aging. Their activity requires the maintenance of an adequate intracellular NAD+...

Frost, M., 1971: This is progress in psychiatry. Nursing Mirror and Midwives Journal 132(9): 42-43

Morozov, A.B.; Tokarev, O.P., 1982: Scientifico-methodological prerequisites for organizing the early detection of hearing disorders in children. Vestnik Otorinolaringologii: 26-30

Islam, S.; Carmen, R.C.; Garner, J.O.J., 2007: Fatty acid compositions in ungerminated (whole seed), cotyledon and embryo tissues of cowpea (Vigna unguiculata L. Walp) seed grown under different temperatures. Cowpea seed germination is affected by temperature and its fatty acid composition. Trials over two years were conducted using 25 cowpea cultivars/genotypes to screen for germination potentiality at 10, 30 and 40 degrees C. Three cultivars were ch...

Jahnen-Dechent, W.; Ketteler, M., 2012: Magnesium basics. As a cofactor in numerous enzymatic reactions, magnesium fulfils various intracellular physiological functions. Thus, imbalance in magnesium status-primarily hypomagnesaemia as it is seen more often than hypermagnesaemia-might result in unwanted n...

Bohadana, A.B.; Massin, N.; Wild, P.; Kolopp, M.N.; Toamain, J.P., 1994: Respiratory symptoms and airway responsiveness in apparently healthy workers exposed to flour dust. Our aim was to measure the levels of exposure to wheat flour dust in a modern industrial bakery, and to assess the relationship between respiratory symptoms, sensitization to wheat flour antigens and airway responsiveness in the work force. Forty...

Sukovatykh, B.S.; Blinkov, I.Iu.; Eshtokin, S.A.; Frolova, O.G., 2009: Experimental-clinical grounds of the application of immobilized forms of sodium hypochlorite in treatment of diffuse peritonitis. Experimental (200 experiments) and clinical (in 46 patients) investigations have shown that immobilized forms of sodium hypocloride in methylcellulose gel makes the lethality level 1.9 times lower and the formation of abdominal adhesions 1.8 times...