+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

tRNA anticodon replacement experiments show that ribosomal frameshifting can be caused by doublet decoding

, : tRNA anticodon replacement experiments show that ribosomal frameshifting can be caused by doublet decoding. Proceedings of the National Academy of Sciences of the United States of America 83(14): 5062-5066

The expression of certain normal genes requires a specific ribosomal frameshift event because the mRNA has the coding information for one protein in two different reading frames. One of several possible mechanisms for this involves recognition of a nontriplet codon by a noncognate tRNA. The AGUC-decoding Escherichia coli tRNASer3 reads a GCA alanine codon to cause a -1 frameshift. Replacement of the anticodon of tRNAPhe with the anticodon of tRNASer3 allows the constructed tRNA to cause this frameshifting. By altering the anticodon loop nucleotides at positions 33-36 in the constructed tRNAPhe molecules, the tRNA was found to recognize a 2-base codon. Instead of the usual anticodon, positions 34-36, the nucleotides in positions 34 and 35 form essential base pairs with the first two positions of the alanine codon. The uridine in position 36 is also required but not for base pairing.


PMID: 2425361

Other references

Corcoran, M.S.; van Loo, I.H.M.; Wolffs, P.F.G.; van Well, G.Th.J., 2015: Interpretation of real-time PCR diagnosing fecal viruses in children: a prospective case-control study. Pediatric Infectious Disease Journal 32(12): 1402-1403

Okayama, S.; Uemura, S.; Nishida, T.; Morikawa, Y.; Onoue, K.; Takemoto, Y.; Soeda, T.; Somekawa, S.; Ishigami, K-ichi.; Takeda, Y.; Horii, M.; Kawata, H.; Takaoka, M.; Nakajima, T.; Saito, Y., 2008: Progression of non-culprit coronary artery atherosclerosis after acute myocardial infarction in comparison with stable angina pectoris. We previously found, using a mouse model, that activation of proinflammatory cytokines after acute myocardial infarction (AMI) augments neointimal hyperplasia of a remote artery. The present study assessed the progression of luminal narrowing of n...

Dugois, P.; Chatard, H.; Colomb, L.; Gagnaire, J., 1959: A case of Pautrier-Woringer lipo-melanic reticulosis. Bulletin de la Societe Francaise de Dermatologie et de Syphiligraphie 5: 731-732

Kelley, T.J.; Cotton, C.U.; Drumm, M.L., 1998: Regulation of amiloride-sensitive sodium absorption in murine airway epithelium by C-type natriuretic peptide. We have previously shown that C-type natriuretic peptide (CNP), a guanylate cyclase agonist, can stimulate cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride secretion in murine airway epithelial cells via protein kinase...

Ellis,S.B.; Appenzeller,G.; Lee,H.; Mullen,K.; Swenness,R.; Pimentel,G.; Mohareb,E.; Warner,C., 2008: Outbreak of sandfly fever in central Iraq, September 2007. An outbreak of nonspecific febrile illnesses occurred among U.S. Army troops in September 2007 at a remote, newly established, rural-situated patrol base, south of Baghdad, Iraq. Soldiers displayed an acute flu-like syndrome with symptoms of fever...

Greenland, P.; Grundy, S.; Pasternak, R.C.; Lenfant, C., 1998: Problems on the pathway from risk assessment to risk reduction. Circulation 97(18): 1761-1762

Malan C.E.; Gandini A., 1966: Yeasts from the source of nourishment the pedotrophic nest and the digestive tract of the larvae of dung eating scarabs chironitis furcifer scarabaeus semipunctatus pichia polymorpha sporobolomyces salmonicolor rhodotorula sp torulopsis candida candida humicola trichosporon cutaneum. Atti della Accademia delle Scienze di Torino I Classe di Scienze Fisiche Matematiche e Naturali 100(1): 97-118, 1965

Davila, A.F.; McKay, C.P., 2015: Chance and necessity in biochemistry: implications for the search for extraterrestrial biomarkers in Earth-like environments. In this paper, we examine a restricted subset of the question of possible alien biochemistries. That is, we look into how different life might be if it emerged in environments similar to that required for life on Earth. We advocate a principle of...

Korhonen, J., 1959: Chronic essential hypochromic anemia in 3 male patients. Duodecim; Laaketieteellinen Aikakauskirja 75: 716-719

Ramana G.V.; Reddy B.B.; Reddy K.K.; Murthy A.S.N.; Haranath G.B., 1989: Effect of prostaglandins on characteristics of buffalo semen at 4c. The effect was studied of exogenously added prostaglandins (PGs) E2 and/or F2.alpha. at different concentrations on certain characters of diluted semen of Murrah buffalo breeding bulls at 0, 24, 48 and 72 h of incubation. It was observed that the...