+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

3H-vasopressin binding to the rat mesenteric artery

, : 3H-vasopressin binding to the rat mesenteric artery. Endocrinology 113(1): 409-411

We investigated the binding of 3H-Arg8-vasopressin to membranes from rat mesenteric arteries. Specific binding of 3H-vasopressin was 60-75% of total binding. Binding at 22 C achieved a plateau at 30 min whereas at 4 C binding was significantly slower. Binding was reversible upon addition of 1 microM Arg8-vasopressin after 30 min of incubation. Scatchard analysis indicated a single class of high-affinity binding sites with an equilibrium dissociation constant of 5.1 +/- 0.6 nM and a total binding capacity of 91 +/- 12 fmol/mg protein. Competitive inhibition of 3H-Arg8-vasopressin binding showed an IC50 of 3 nM for Arg8-vasopressin, 14 nM for [I-(beta-mercapto-beta-beta-cyclopentamethylene-propionic, 4-valine, 8-D-arginine]-vasopressin, 31 nM for oxytocin, 52 nM for I-deamino-8-D-arginine-vasopressin, 0.1 microM for [I-deamino-penicillamine, 4-valine, 8-D-arginine]-vasopressin, and 0.8 microM for desglycinamide-deamino-Arg8-vasopressin. Unrelated peptides did not displace 3H-Arg8-vasopressin. We conclude that these binding sites possess characteristics of physiologically relevant vasopressin receptors in vascular smooth muscle of a resistance type vessel.


PMID: 6861711

DOI: 10.1210/endo-113-1-409

Other references

Tokunaga, T.; Morishita, H.; Kushiku, K.; Abe, M.; Matsuki, J.; Inoue, T.; Furukawa, T., 1983: Pharmacodynamic effects of zopiclone. Igaku Kenkyu. Acta Medica 53(4): 224-232

Bidwell, G.L.; Raucher, D., 2011: Cell penetrating elastin-like polypeptides for therapeutic peptide delivery. Current treatment of solid tumors is limited by side effects that result from the non-specific delivery of drugs to the tumor site. Alternative targeted therapeutic approaches for localized tumors would significantly reduce systemic toxicity. Pept...

Anonymous, 1984: Other factors to consider in infantile AIDS. New England Journal of Medicine 311(3): 189-191

Michele, D.E.; Coutu, P.; Metzger, J.M., 2002: Divergent abnormal muscle relaxation by hypertrophic cardiomyopathy and nemaline myopathy mutant tropomyosins. Mutations in tropomyosin (Tm) have been linked to distinct inherited diseases of cardiac and skeletal muscle, hypertrophic cardiomyopathy (HCM), and nemaline myopathy (NM). How HCM and NM mutations in nearly identical Tm proteins produce the vastl...

Levin, O.S.; Batukaeva, L.A.; Anikina, M.A.; Iunishchenko, N.A., 2010: Efficacy and tolerability of choline alphoscerate (cereton) in patients with Parkinson's disease with cognitive disorders. An open 10-day study of treatment effect of cereton in comparison to piracetam has been conducted. Cereton was used in 40 patients (main group) in dosage 1000 mg, piracetam - in 20 patients (control group) in dosage 2000 mg. Both drugs were used i...

Walzer, P.D.; LaBine, M.; Redington, T.J.; Cushion, M.T., 1984: Predisposing factors in Pneumocystis carinii pneumonia: effects of tetracycline, protein malnutrition, and corticosteroids on hosts. Components of the immunosuppressive regimen used to reactivate latent Pneumocystis carinii infection were analyzed for their effects on the growth, nutrition, and lymphoid system of hosts. Rats that were administered either tetracycline or a low-p...

Ismail, A.; Ktari, L.; Ahmed, M.; Bolhuis, H.; Boudabbous, A.; Stal, L.J.; Cretoiu, M.Silvia.; E.B.ur, M., 2016: Antimicrobial Activities of Bacteria Associated with the Brown Alga Padina pavonica. Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by se...

Angulo, A.O.; Rodriguez, M.A.; Olivares, T.S., 1999: A new genera and new species of Hight Andean III Faronta Smith, 1908 v/s Strigania Hampson, 1905 and an approach to Dargida Walker, 1856 Lepidoptera Noctuidae Nuevo genero y nuevas especies de noctuidos altoandinos III Faronta Smith, 1908 v/s Strigania Hampson, 1905 y algunos alcances acerca de Dargida Walker, 1856 Lepidoptera Noctuidae. Gayana Zoologia, 632: 67-85

Lukas, K.H., 1957: Psychological methods of facilitation of labor. Geburtshilfe und Frauenheilkunde 17(9): 765-774

Markman, M., 2003: Managing taxane toxicities. The two commercially available taxanes (paclitaxel and docetaxel) are widely employed in standard oncologic practice. Toxicity of the agents includes bone marrow suppression (principally neutropenia), complete alopecia, and hypersensitivity reacti...