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A new method of ovulation induction with HMG-HCG by a E2 rapid radioimmunoassay

, : A new method of ovulation induction with HMG-HCG by a E2 rapid radioimmunoassay. Nihon Sanka Fujinka Gakkai Zasshi 34(1): 11-20

We schemed out a new method of ovulation induction with HMG-HCG using E2 rapid radio-immunoassay (RIA). On measurement of E2 values, the method of E2-(125)I kit (Daiichi Radioisotope Labs., LTD) was simplified, that is, an omission of defatting, shortened incubation time and a standard curve constructed by three points. There was a good correlation between the E2-(125)I kit method and the E2 rapid RIA method in E2 values obtained. The lowest detectable amount was 75 pg/ml. This rapid method seemed to be suitable to monitor the extent of follicular maturation during HMG therapy. Our new method of ovulation induction was as follows. (i) On the basis of E2 values measured every 3 days, HMG dosage was adjusted. (ii) When E2 was over 400 pg/ml for 2 days, HCG was given on the next day. with this method, there was an increase in the percentage of ovulatory cycles from 54.5% to 100% and a decline in the incidence of ovarian enlargement and the ascites. This new method minimized inconvenience and expense of E2 determination without drastically influencing therapy outcome.


PMID: 6801156

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