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A new method of the measurement of the electrically neutral fluxes of cations through lipid bilayer membranes induced by Men+/nH+-exchangers


, : A new method of the measurement of the electrically neutral fluxes of cations through lipid bilayer membranes induced by Men+/nH+-exchangers. Febs Letters 163(1): 42-45

Electrically neutral ionophores (nigericin, monencin) incorporated into a planar bilayer lipid membrane (BLM) bring about hydrogen ion gradient formation in the unstirred layers of BLM if a metal ion gradient on the membrane is prepared. Under these conditions a diffusion potential of a hydrogen ion is generated after addition of a protonophore. Cation selectivity of nigericin, monencin and A23187 has been studied by means of electrical potential measurements in the presence of a protonophore and Men+/nH+-exchangers mentioned above. The data on cation selectivity are in a good agreement with the well known results of the direct measurements of metal ion fluxes. This shows that the effect of generation of the potential on BLM in the presence of a protonophore and a Men+/nH+-exchanger can be used for the estimation of electrically neutral ion fluxes through BLM.

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PMID: 6414844


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Kalani, M.Yashar.S.; Wanebo, J.E.; Martirosyan, N.L.; Nakaji, P.; Zabramski, J.M.; Spetzler, R.F., 2017: A raised bar for aneurysm surgery in the endovascular era. Journal of Neurosurgery 126(5): 1731-1739

Pretorius S.J., 1988: Freshwater mollusc studies a personal experience. My contribution reviews some of the research activities of the Snail Research Unit. Taking Unit. Taking some liberties on the chronological order, the transition is traced from the species descriptions of the early years, the extensive field surve...

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Sota T., 1988: Univoltine and bivoltine life cycles in insects a model with density dependent selection. Selection for univoltine and bivoltine life cycles in insects under resource-limited but favourable temperature conditions is analyzed with a difference equation model including density-dependent population dynamics based on the conceptual framewo...

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Buckley, J.J.C., 1938: On Culicoides as a vector of Onchercerca gibsoni. A critical examination of the literature on O. gibsoni in Australian cattle and on other spp. of Onchocerca in different parts of the world suggested that the probable intermediate host of O. gibsoni would be found among members of the Nematocerca...

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Levine, L., 2004: Proteasome inhibitors: their effects on arachidonic acid release from cells in culture and arachidonic acid metabolism in rat liver cells. Background: I have postulated that arachidonic acid release from rat liver cells is associated with cancer chemoprevention. Since it has been reported that inhibition of proteasome activities may prevent cancer, the effects of proteasome inhibito...

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