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A new method that investigates superoxide versus respiration in vitro using bioluminescence and Sepharose-bound adenosine derivatives


, : A new method that investigates superoxide versus respiration in vitro using bioluminescence and Sepharose-bound adenosine derivatives. Journal of Biochemical and Biophysical Methods 6(2): 81-87

In vitro reactions between superoxide and phosphate derivatives of adenosine yielded quantitative amounts of stable ozonide-products. ADP-ozonide was formed in an optimized in vitro synthesis from ADP + O-(2) and affected by inhibitors and uncouplers in a similar manner to in vivo, oxidative-phosphorylation results. ADP-ozonide was further reacted with phosphoric acid to form ATP. Superoxide and ADP-ozonide may be important carrier-intermediates between respiration's electron-transport chain and nodule ATP formation in vivo.

US$29.90

PMID: 6286748


Other references

Warland, J.S.; Thurtell, G.W., 2000: A micrometeorological method for in situ denitrification measurements using acetylene inhibition. A method for acetylene inhibition measurements of denitrification in the field using micrometeorological techniques is presented. It is based on the monitoring of flux ratios from small (<5 msuperscript 2) microplots. The method measures concen...

R.B.R.ge; A.G.dio, 2012: Multimodal inversion of guided waves in georadar data. We analyze the dispersive characteristics of the electromagnetic guided waves (at georadar frequency) to infer the electrical properties of materials that constitute a layered wave guide.WARR (Wide Angle Reflection and Refraction) georadar acquisi...

Zuo, G-Ying.; Meng, F-Yan.; Han, J.; Hao, X-Yan.; Wang, G-Chun.; Zhang, Y-Ling.; Zhang, Q., 2011: In vitro activity of plant extracts and alkaloids against clinical isolates of extended-spectrum β-lactamase (ESBL)-producing strains. The antibacterial activity of 80% ethanol extracts of 10 medicinal plants collected in Yunnan (Southwest China), was tested against clinical isolates of extended-spectrum β-lactamase (ESBL)-producing strains. Their MIC values ranged between 1.56-...

Blinov, B.V., 1970: Reconstructive-restorative surgery on the hip joint using homoplasty of the trochanteric fossa. Ortopediia Travmatologiia i Protezirovanie 31(10): 26-31

Gamova, I.A.; Sidorenko, V.D.; Nosova, M.V.; El' bert, A.A., 1990: Modification of wood to obtain pressed items. Chips of Scots pine [Pinus sylvestris] and aspen [Populus tremula] were mixed with an adhesive and used to made extruded rods in a screw-drive extrusion installation. Various chip pretreatments were compared, viz, steaming for 1 hour at 120, 140 a...

Bakhur, V.T., 1974: "Mixed" strokes as one of the forms of complicated acute cerebral circulatory disorders. Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 74(6): 809-814

Noel, J.; Roux, D.; Pouyssegur, J.; Claveau, D.; Nault, G., 1997: Sorting determinant for membrane expression of Na-H exchangers in polarized OK cells. Journal of the American Society of Nephrology 9(PROGRAM AND ABSTR ISSUE): 63A, Sept

Potapov, A.P., 1985: Mechanism of the stereospecific stabilization of codon-anticodon complexes in ribosomes during translation. Zhurnal Obshchei Biologii 46(1): 63-77

Thomas, L.A.; Gorzalka, B.B., 2013: Effect of sexual coercion proclivity, insult and fantasy on emotional reactivity and appeal of sexual aggression. Fifty-nine heterosexual university males were assessed for Sexual Coercion Proclivity (SCP) and randomly assigned to one of three conditions: Insult/nonsexually coercive fantasy material; no insult/sexually coercive fantasy material; or, insult/se...

Fu, X.; Shen, Z.; Chen, Y.; Xie, J.; Guo, Z.; Zhang, M.; Sheng, Z., 1998: Randomised placebo-controlled trial of use of topical recombinant bovine basic fibroblast growth factor for second-degree burns. Background: Wound healing is a dynamic process that could be accelerated by growth factors. We investigated the effect of recombinant bovine basic fibroblast growth factor (rbFGF) on burn healing in a randomised placebo-controlled trial. Methods:...