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A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells

, : A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells. Biochemical and Biophysical Research Communications 44(2): 513-519


PMID: 5159785

Other references

Lindsay, D.T., 1949: Control of human epiphyseal growth. Bulletin of the University of Minnesota Hospitals and Minnesota Medical Foundation. University of Minnesota. Hospitals 20(23): 450-469

Virstiuk, N.H., 1997: English walnuts in the combined treatment of rheumatoid arthritis patients. Likars'ka Sprava: 123-126

Waki, A.; Kato, H.; Yano, R.; Sadato, N.; Yokoyama, A.; Ishii, Y.; Yonekura, Y.; Fujibayashi, Y., 1998: The importance of glucose transport activity as the rate-limiting step of 2-deoxyglucose uptake in tumor cells in vitro. Glucose transporter (GLUT) expression and hexokinase activity are thought to be related to high (18F)-fluorodeoxyglucose (FDG) uptake in tumor cells, but their relative importance is still unknown. To determine which is the predominant factor in F...

Das, B.C.; Bujarbaruah, K.M.; Sanyal, S.; Das, P.K.; Ghosh, P.R.; Naskar, S.; Khan, M.H.; Das, A.B.; Bandopadhyay, S., 2006: Morphological characteristics of boar spermatozoa preserved with BTS diluent. BTS extender was used for the preservation of boar spermatozoa at different hours. Morphological characteristics (motility, live percentage and acrosomal integrity) were studied before and after preservation, because these are the most important f...

Morishima, Y.; Chida, K.; Muroya, Y.; Utsumi, Y., 2017: Effectiveness of a New Lead-Shielding Device and Additional Filter for Reducing Staff and Patient Radiation Exposure During Videofluoroscopic Swallowing Study Using a Human Phantom. Interventional radiology procedures often involve lengthy exposure to fluoroscopy-derived radiation. We therefore devised a videofluoroscopic swallowing study (VFSS) procedure using a human phantom that proved to protect the patient and physician...

Delsate D.; Henrotay M.; Godefroit P., 1991: Occurrence of fossil insects in the lower Toarcian of Belgium. Bulletin de la Societe Belge de Geologie = Bulletin van de Belgische Vereniging voor Geologie 100(1-2): 147-153

Klop, B.; Kock, M.C.J.; Vincenten, J.P.L.; Kofflard, M.J.M., 2017: An undifferentiated pulmonary carcinoma growing into the left atrium causing cardiac embolism. International Journal of Cardiovascular Imaging 33(6): 915-916

Klaassens, E.R.; van Veen, T.; Zitman, F.G., 2007: Does trauma cause lasting changes in HPA-axis functioning in healthy individuals?. Although the majority of people who are exposed to traumatic events do not develop psychopathology, trauma has often been associated with increased vulnerability to psychiatric disorders. In addition, alterations in the HPA-axis have been demonstr...

Modiaev, V.P., 1980: Structure and function of the fibrous structure of articular cartilage. Structure of the articular cartilage fibrous stroma in the mature rabbit femoral heads has been studied and described by means demasking of fibres, of electron microscopy and polarization microscopy. A new scheme on the articular cartilage fibrous...

Hoffman, J.C.; Vaughn, K.C., 1994: Griseofulvin destabilizes plant microtubules. Griseofulvin is a weak microtubule-destabilizing agent when tested in mammalian cells and in in vitro assays of polymerization of brain tubulin. Observed effects of griseofulvin on plant cells have thus far been limited to microtubule organizing c...