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A new method which investigates lysosomal sensitivity to superoxide with endocytotic, site-specific, chemiluminescent probes


, : A new method which investigates lysosomal sensitivity to superoxide with endocytotic, site-specific, chemiluminescent probes. Mechanisms of Ageing and Development 17(3): 275-281

The end result of cellular autophagocytosis and lysosomal digestion is incompletely understood in regard to the roles of the superoxide anion radical (O2-.). Cultured glial cells sequestered endocytotically two probes that were site-specific to the lysosome vacuome and sensitive to free-radical activities. The Sepharose-4B-polyisoluminol probe emitted chemiluminescent light in proportion to externally injected O2(-).. Bioluminescence measurements of beta-glucuronidase activity in intact glial cell lysosomes was achieved with the second site-specific, enzyme-specific Sepharose-4B-(dodecanate)5'-luciferinylglucuronate probe. Considerable activity was found in the lysosomal vacuome. In conclusion, we suggest that the use of site-specific, chemiluminescent probes can be of importance in the study of free radicals.

US$29.90

PMID: 6275214


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