+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new methodological approach for studying axonal transport: cytofluorometric scanning of nerves

, : A new methodological approach for studying axonal transport: cytofluorometric scanning of nerves. Journal of Histochemistry and Cytochemistry 32(1): 7-16

A new technique for studying axonal transport has been developed. The technique, which is based on histofluorescence techniques, enables the measurement of several different accumulated substances and parameters within a single nerve in relation to a nerve crush or local cooling. Any substance that can be made to fluoresce can be measured. The tissue is treated according to the formaldehyde-induced fluorescence method of Hillarp and Falck for visualization of monoamines, or according to the indirect immunofluorescence method. For immunofluorescence the nerve is cryostat-sectioned and various sections can be incubated with primary antisera against different antigens. After incubation and mounting the sections are placed in a cytofluorimeter (Leitz MPV II). They are passed under a measuring slit at a steady speed by a motor driven cross-table. The fluorescence intensity passing through the measuring slit is continuously registered by a recording unit with an integrator. This recorder produces a graphical nerve accumulation profile, and the area under the profile, relating to the fluorescence, is expressed in arbitrary units. This article presents data on the accumulation of noradrenaline, dopamine beta-hydroxylase, and tyrosine hydroxylase in crush-operated rat sciatic nerve. The time-course accumulations for noradrenaline (visualized by the Falck and Hillarp method) and dopamine beta-hydroxylase (visualized by immunofluorescence) demonstrated a striking similarity, which is to be expected since the two substances are stored in the same organelle. Tyrosine hydroxylase (visualized by immunofluorescence) showed a slower accumulation with time, but faster than would be expected had the enzyme been 100% soluble. Colchicine but not lumi-colchicine blocked the transport of noradrenaline organelles. With the new scanning technique we have the potential to study accumulation profiles of several different substances within a single nerve. Morphometric data, morphological observations, and photograph documentation of the same nerve section are also available.


PMID: 6197439

Other references

January, L.E., 1972: Heart disease in the world: prospects for prevention. Indian Heart Journal 24(2): 87-91

Pizzi de Parra, N.; Larralde de Luna, M.; Cicioni, V.; Parra, C.A., 1987: Infantile acropustulosis. Considerations on 11 cases. Eleven patients with infantile acropustulosis, 10 from Mendoza and one from Buenos Aires, are presented. Remarkably was a blood eosinophilia in 4 cases and the prompt improvement with dapsone in 2 cases. The differential diagnosis with other derma...

Nir, C.C.; Floiras, J.L., 1992: Role of radiotherapy in the treatment of bone metastases. The most frequently encountered metastases during evolution of cancers are bone metastases, which involve painful syndrome. External radiotherapy is an effective and indisputable treatment to give relief to suffering patients. The modalities of th...

Lundberg P.O.; Muhr C.; Hartvig P.; Bergstrom K.; Lindberg B.; Jung B.; Lundqvist H.; Langstrom B.; Stalnacke C.G., 1983: Pharmaco kinetics of carbon 11 labeled bromocriptine in rhesus monkey studied by positron emission tomography. Acta Endocrinologica Supplementum 103(256): 85

Bethke, R.M.; Record, P.R.; Kennard, D.C., 1933: Relation of vitamin G complex to hatchability and nutritive value of eggs. The vitamin G (B2) complex was shown to have a beneficial effect on hatchability. The vitamin B2potency of eggs from differently fed hens varied, as shown by growth tests on chicks and rats. The growth response could be correlated with the hatchab...

Wellman L.; Jacobson A., 1976: Intra amniotic prostaglandin and urea for mid trimester abortion. Fertility & Sterility 27(2): 218

Antonovitz, F.R.e, T., 1984: A theoretical and empirical approach to the value of information in risky markets. Staff paper P University of Minnesota Dept of Agricultural and Applied Economics: 984 (84-28)

Heilbronn, Alfred, 1929: Development of HCN in ferns. Polypodium aureum (forma triploidea) after exposure to a very low temp. developed a large amount of HCN. The presence of the acid was proved with Na picrate and with the blue precipitate formed on addition of FeSO4 to an alkaline solution, boiling...

Hamaguchi, S.; Sakaizumi, M., 1992: Sexually differentiated mechanisms of sterility in interspecific hybrids between Oryzias latipes and O. curvinotus. The fertility of hybrids between the medaka Oryzias latipes and O. curvinotus was examined. F1 [female][female] were able to lay eggs, but [male][male] were sterile. Histological examination of the ovaries of hybrids revealed that oogenesis did no...

Rudali, G.; Guggiari, M., 1974: Carcinogenic effect of two norgestrels on mouse mammary gland. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 168(10-11-12): 1190-1194