geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new micromethod for the quantification of low molecular weight oligomers of polyethylene glycol


, : A new micromethod for the quantification of low molecular weight oligomers of polyethylene glycol. Clinica Chimica Acta; International Journal of Clinical Chemistry 134(3): 245-254

We describe a gas-liquid chromatographic technique for quantifying the low molecular weight (Mr 106-634) oligomers of polyethylene glycol (PEG) in clinical specimens. The deionized sample, containing tetra-ethylene glycol as an internal standard, is applied on column. This technique readily quantifies as little as 2.5 micrograms of an individual oligomer; with such a quantity, the coefficient of variation is +/- 2.5 percent (N = 25 analyses). Small volumes (250 microliter) of urine are conveniently analyzed, and a single column can be utilized for the analysis of approximately three hundred specimens. We have analyzed timed urine specimens from humans who received 0.15 g of PEG 400 per kilogram. Individuals varied markedly with regard to the total amount of PEG excreted into the urine; each subject, however, consistently excretes a uniform percentage of the ingested dose. The urinary oligomeric profile of PEG does not vary from subject to subject nor from hour to hour, during the first six hours following oral administration, so that a random urine obtained during this period provides a reliable clinical specimen. This technique should facilitate clinical studies that utilize polyethylene glycol 400 as an index of passive intestinal transport.

US$29.90

PMID: 6640945


Other references

Güler, G.; Atalay, N.S., 1996: Changes in hydroxyproline levels in electric field tissue interaction. Guinea pigs when exposed to external electric field (1.9 kV/m) for 9 hrs/day for 3 days registered rise in the levels of hydroxyproline in liver, lung and kidney, suggesting increased synthesis of collagen.

Ko, C.; Kaushal, A.; Hammoud, D.A.; Steffen-Smith, E.A.; Bent, R.; Citrin, D.; Camphausen, K.; Warren, K.E., 2012: Role of early postradiation magnetic resonance imaging scans in children with diffuse intrinsic pontine glioma. To determine optimal timing of assessing postradiation radiographic response on magnetic resonance imaging (MRI) scans in pediatric patients with diffuse intrinsic pontine glioma (DIPG). Patients were treated on a prospective study at the National...

Anonymous, 2011: Toxicity of essential and non-essential oils against the chewing louse,BovicolaWerneckiellaocellatus. The toxicity of six plant essential oils to the chewing louse,Bovicola(Werneckiella)ocellatuscollected from donkeys, was examined in laboratory bioassays. The oils examined were: tea-tree (Melaleuca alternifolia), lavender (Lavandula angustifolia)...

García-Cruz, D.; Rivera, H.; Barajas, L.O.; Jiménez-Sáinz, M.; Nazará, Z.; Sánchez-Corona, J.; Durón-Huerta, H.; García-Ochoa, C.; Cantú, J.M., 1985: Monosomy 20p due to a de novo del(20)(p12.2). Clinical and radiological delineation of the syndrome. A 16 year-old boy with monosomy 20p was studied. The clinical and radiological data compared with those from the three previously reported cases, permit the delineation of a distinct syndrome of low birthweight, flat face, low nasal bridge, long p...

Schluesener H.; Lee S.J.; Weiner H.L., 1987: Conservation of t cell receptor beta chain gene rearrangement patterns in autoimmune t cells during chronic relapsing experimental allergic encephalomyelitis eae. Federation Proceedings 46(4): 1376

Zubovskii G.A.; Khokhlova L.T.; Milyagin V.A., 1980: Comparative analysis of the absorptive excretory function of the normal liver with iodine 131 labeled bromsulfan and bengal rose. A comparative analysis was made of the absorptive-excretory function of normal [human] tissue with 131I-labeled bromsulfan and Bengal rose. Using the 2 radiopharmaceutical preparations, the activity curves of the blood, liver and the intestine hav...

Nast-Kolb, D.; Taeger, G.; Bardenheuer, M., 2000: Limited surgical indications for elderly and multi-morbid patients. Der Unfallchirurg 103(2): 168-171

Falsey, A.R., 2012: Current management of parainfluenza pneumonitis in immunocompromised patients: a review. Parainfluenza viruses (PIV) are common respiratory viruses that belong to the Paramyxoviridae family. PIV infection can lead to a wide variety of clinical syndromes ranging from mild upper respiratory illness to severe pneumonia. Severe disease ca...

Fishman, A., 2011: The effects of parity, breastfeeding, and infertility treatment on the risk of hereditary breast and ovarian cancer: a review. Knowledge of the potential association of parity, breastfeeding, and infertility treatment on breast and ovarian cancer risk for BRCA1 and BRCA2 mutation carriers is important and should be a crucial part of genetic counseling. The discussion of p...

Giraud, G.; Latour, H.; Levy, A.; Puech, P., 1955: Treatment of acute articular rheumatism after its acute phase. Montpellier Medical 48(3): 339-344