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A new microspectrophotometric method for measuring absorbance of rat photoreceptors

, : A new microspectrophotometric method for measuring absorbance of rat photoreceptors. Vision Research 24(11): 1673-1676

A method has been developed for obtaining visual pigment absorbance spectra from fixed, frozen sections of the albino rat retina. The retinas are transversely sectioned at various thicknesses on a cryo-microtome and the resulting slices are examined with a photon-counting microspectrophotometer. Problems caused by the limited pigment content of small receptors are reduced and measurements can be made at well-specified locations along the retinal section. This method is both precise and accurate and permits comparisons across sections and across animals.


PMID: 6533992

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