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A new mobilizable cosmid vector for use in Vibrio cholerae and other gram-negative bacteria

, : A new mobilizable cosmid vector for use in Vibrio cholerae and other gram-negative bacteria. Gene 153(1): 85-87

A new mobilizable cosmid vector, pCOS5, was engineered for use in Vibrio cholerae (Vc). Plasmid pCOS5 is small in size (7286 bp), contains the oriT from plasmid RK2, and has several unique restriction sites. The complete nucleotide sequence of pCOS5 was deduced from the DNA fragments used in its construction. Biparental matings using Escherichia coli (Ec) SM10 and triparental matings using Ec DH5 alpha[pRK2013] were used to measure the conjugation frequency of pCOS5 and pAJM1, a clone containing a 40-kb insert of chromosomal DNA from Vc ligated into pCOS5. Transfer of pCOS5 or pAJM1 to Vc occurred at a frequency of between 10(-2)-10(-3) transconjugants per recipient cell. The promiscuous nature of RP4/RK2 transfer functions makes pCOS5 a potentially useful vector for mobilizing large fragments of cloned DNA between different Gram- bacteria that support replication of ColE1 plasmids or as a mobilizable suicide vector in Gram- bacteria where replication of ColE1 plasmids is not supported.


PMID: 7883191

Other references

Weigend, S.R.manov, M., 2002: The World Watch List for Domestic Animal Diversity in the context of conservation and utilisation of poultry biodiversity. World's poultry science journal 58(4): 411-430

Morán Pinto, O., 1973: Evaluation of the new therapy in the treatment of shock. Use of the beta adrenergic metaproterenol. Revista Espanola de Anestesiologia Y Reanimacion 20(4): 486-495

Jolly, C.J., 1963: A suggested case of evolution by sexual selection in primates. Man, 63222: 177-178

Hochstein, P.; Cohen, G., 1962: The action of hydrogen peroxide on tumor cells. Proc Amer Assoc Cancer Res 3(4): 329

Nymberg, J.H.; Van Noppen, B., 1994: Obsessive-compulsive disorder: a concealed diagnosis. Epidemiologic data suggest that obsessive-compulsive disorder is the fourth most common mental disorder, after phobias, substance abuse and depression, and is nearly as common as asthma and diabetes mellitus. The understanding of obsessive-compuls...

Rabinow, J., 1989: Where you stand in the eyes of the law (continuing education credit). Nursing 19(2): 34-42

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Kao, Y.H.; Sorenson, J.A.; Winkler, S.S., 1999: Comment on "Cerebral tumor volume calculations using planimetric and eigenimage analysis" [Med. Phys. 23, 2035-2042 (1996). Medical Physics 25(12): 2478-2479