geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new mouse cell-surface antigen (Ly-m20) controlled by a gene linked to Mls locus and defined by monoclonal antibodies


, : A new mouse cell-surface antigen (Ly-m20) controlled by a gene linked to Mls locus and defined by monoclonal antibodies. Immunogenetics 14(1-2): 3-14

Five monoclonal antibodies were established by the fusion of mouse myeloma cells (NS.1) with spleen cells from A and (A x C3H/An)F1 mice hyperimmunized with 70Z/3 tumor cells. These antibodies recognized a new antigenic specificity provisionally called Ly-m20.2. In direct cytotoxicity assays, 60 percent of cells in spleen, 40 percent in lymph node, 50 percent in bone marrow and less than 5 percent in thymus were found to react with three of the five antibodies, whereas the two others yielded somewhat lower cytotoxicity indices. The Ly-m20.2 antigen was also expressed on cells derived from liver and kidney but not on cells derived from brain. As judged from cytotoxicity assays with separated T and B cells, Ly-m20.2 antigen is carried preferentially on B lymphocytes. Direct plaque-forming cells (PFC) were completely eliminated by Ly-m20.2-specific antibody and complement. Linkage tests by analysis in 20 (CBA/J x C3H/An) x C3H/An backcross mice and by segregation analysis of BXH and SWXL recombinant inbred strains indicate close association of the loci controlling Ly-m20.2 and M1s antigens on chromosome 1.

US$29.90

PMID: 6173319


Other references

Petersen, J.; Ritzau-Tondrow, U., 1988: Chronic glaucoma following silicone oil implantation: a comparison of 2 oils of differing viscosity. Fortschritte der Ophthalmologie 85(6): 632-634

Sohn H.J.; Kim E.S.; Kim S.S., 1985: Chemical evidence for identical subunits of tobacco nicotiana tabacum 6 phosphogluconate dehydrogenase band ii. 6-Phosphogluconate dehydrogenase band II isoenzyme was isolated from a tobacco callus culture line, Nicotiana tabacum L., var. Virginia 115, utilizing ammonium sulfate precipitation, DEAE-cellulose chromatography, Red Sepharose CL-6B affinity chro...

Zuffa, M.; Messerschmidt, I., 1973: Acute lumboischialgic syndrome simulating vascular occlusion of the lower extremity. Bratislavske Lekarske Listy 59(4): 496-498

Daniloff, R.G.; Shriner, T.H.; Zemlin, W.R., 1968: Intelligibility of vowels altered in duration and frequency. Journal of the Acoustical Society of America 44(3): 700-707

Rocha, G.; Baines, M.G.; Deschenes, J.; Duclos, A.; Antecka, E.; D.S.lvio, M.; Burnier, M., 1994: Relationship between transforming growth factor-beta and nitric oxide during experimental uveitis. Investigative Ophthalmology & Visual Science 35(4): 1686

Yaguchi, T.; Someya, A.; Udagawa, S.I.hi, 1996: New and rare microfungi from the island of Hachijo-jima. Two striking fungi, isolated from soils from the island of Hachijo-jima in Japan, are described and illustrated. One of these represents a new species of Talaromyces (Ascomycetes), for which the name Talaromyces hachijoensis is proposed. The new s...

Kretic, M., 1953: The problem of the goiter. Medicinski Arhiv 7(6): 1-27

Dobrovolsku, BV., 1973: P.A. Sviridenko (1893-1971) as entomologist. Entomologicheskoe Obozrenie, 521: 234-237

Anonymous, 1981: Terminology and classification of glacigenic and related deposits. Pages 22-27 1981

Gómez-Benito, M.; Balsas, P.; Bosque, A.; Anel, A.; Marzo, I.; Naval, J., 2005: Apo2L/TRAIL is an indirect mediator of apoptosis induced by interferon-alpha in human myeloma cells. Interferon-alpha (IFN-alpha) is currently used for the therapy of multiple myeloma (MM) though it is only effective in some patients. IFN-alpha induces apoptosis in some MM cell lines and it has been proposed to occur through an autocrine loop inv...