geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new muscle phenotype is expressed by subcultured quail myoblasts isolated from future fast and slow muscles


, : A new muscle phenotype is expressed by subcultured quail myoblasts isolated from future fast and slow muscles. Journal of Biological Chemistry 258(6): 3883-3888

Differentiation of quail myoblasts, isolated from thigh pectoralis and anterior latissimus dorsi muscle, was analyzed in primary cultures and in cultures obtained following repeated subculturing. Our study shows that quail myoblasts can survive many generations without losing their ability to form myotubes. However, during these subcultures the cells progressively express a new phenotype. This phenotype is characterized by a mixture of myosin light chains such that LC1F, LC2F, and LC2S are present in roughly equimolar amounts, each accounting for 25 to 30% of the total light chain synthesis while LC1S accounts for the remaining 10 to 15%, and by a mixture of fast and slow alpha tropomyosin in which alpha S accounts for 10 to 15% of the alpha subunits synthesis. Clonal analysis indicates that all cells in the population express this phenotype which is also characteristic of subcultures obtained from both future fast and slow muscles. Relationships between this phenotype and muscle development are discussed.

US$29.90

PMID: 6687592


Other references

Peerschke, E.I.; Galanakis, D.K., 1983: Binding of fibrinogen to ADP-treated platelets. Comparison of plasma fibrinogen fractions and early plasmic fibrinogen derivatives. Fibrinogen supports platelet aggregation by binding to specific receptors. The importance of the fibrinogen A alpha chain in this hemostatic function is controversial. We found that fibrinogen derivatives, isolated from plasma or obtained after li...

Chichagova O.A.; Cherkinskiy A.Y.; Tolchel nikov Y.S., 1984: Inversion of the radiocarbon age of humus in present-day soil profiles. Doklady Akademii Nauk SSSR 275(5): 1176-1178

Gee, J.; Ding, Q.; Keller, J.N., 2002: Heat shock proteins and proteasome function in neurodegeneration. Current Medicinal Chemistry - Immunology Endocrine & Metabolic Agents 2(3): 195-204, September

Sano, T.; Kovacs, K.; Stefaneanu, L.; Asa, S.L.; Snyder, D.L., 1989: Spontaneous pituitary gonadotroph nodules in aging male Lobund-Wistar rats. Spontaneous pituitary changes with aging were studied in 130 male Lobund-Wistar rats by light and electron microscopy and immunohistochemistry. These 69 healthy rats (6, 18, and 30 months) and 61 moribund rats (17-50 months) had been maintained in...

Tilgner, D.J.; Barylko, P.Kielna, N., 1959: The level of the threshold and the minimum of the gustatory sensitivity. Acta Physiologica Polonica 10: 741-754

Oleson, M.; Heading, C.; Shadick, K.M.; Bistodeau, J.A., 1994: Quality of life in long-stay institutions in England: nurse and resident perceptions. Quality of life (QOL) of older persons living in institutions is a critical consideration in international economic policies and health care decisions. Yet, there is little relevant research to support decisions about culturally acceptable and eff...

Clemons, K.V.; Stevens, D.A., 1997: Treatment of orogastrointestinal candidosis in SCID mice with fluconazole. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 37: 28

Alexander J.E., 1978: Failure of hematopoietic stem cells in the embryonic bursa to migrate to the thymus and differentiate to functional thymus derived cells. Federation Proceedings: 559

Meador, W.E.; Means, A.R.; Quiocho, F.A., 1993: Modulation of calmodulin plasticity in molecular recognition on the basis of x-ray structures. Calmodulin is the primary calcium-dependent signal transducer and regulator of a wide variety of essential cellular functions. The structure of calcium-calmodulin bound to the peptide corresponding to the calmodulin-binding domain of brain calmodu...

Chen, J-Jung.; Lin, W-Jiou.; Liao, C-Hui.; Shieh, P-Chuen., 2007: Anti-inflammatory benzenoids from Antrodia camphorata. Four new compounds, including three new benzenoids, antrocamphin A, antrocamphin B, and 2,3,4,5-tetramethoxybenzoyl chloride, and a new 1,3-dioxolan-2-one derivative, antrodioxolanone, together with 13 known compounds have been isolated from the f...