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A new organic solvent for use in the clearing of tissues. I. Soft tissue histology

, : A new organic solvent for use in the clearing of tissues. I. Soft tissue histology. Anatomical Record 197(3): 283-288

Histosol is a nonflammable solvent mixture of synthetic aromatic hydrocarbons with a flash point of F (tagged closed cup). It has a lower vapor pressure and evaporation rate than other organic solvents, such as xylene, routinely used as clearing and deparaffinizing agents. Both xylene and Histosol clear and deparaffinize soft organ tissues effectively in the preparation of permanently mounted stained slides, but Histosol appears, in many instances, to be the choice solvent: tissues are easier to section; cell borders and cell surface modifications are most distinct; cytoplasmic eosinophilia is more vivid; and nuclear detail is improved. Of prime importance, Histosol is a safer and more efficient solvent for use in histological and pathological laboratories. Rat kidney, lung, digestive, pancreatic and adrenal tissues were examined.


PMID: 6159808

DOI: 10.1002/ar.1091970302

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