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A new test for phagocytosis of human alveolar macrophages in suspension, using 99mTc labelled human serum albumin particles


, : A new test for phagocytosis of human alveolar macrophages in suspension, using 99mTc labelled human serum albumin particles. Anticancer Research 4(4-5): 283-288

Current studies on human alveolar macrophages (HAM phi) indicate that two subpopulations may exist: adherent and nonadherent HAM phi. Most works in the literature were done exclusively on adherent HAM phi. In order to obviate to the loss of nonadherent HAM phi, we elaborated a new test for measuring endocytosis in HAM phi suspensions. According to this method, HAM phi in suspension were allowed to phagocytize millimicrospheres of human serum albumin labelled with 99m Tc (0.2 less than 0 less than 0.5 m; TcK9R - Cis Sorin Biomedica). Bound activity was separated from free activity by centrifugation on PercollR. The resulting internatant ring was found to contain activity bound to HAM phi which had phagocytized human serum albumin millimicrospheres. HAM phi were characterized by morphology and cytochemistry. The method here presented allows the functional study of both adherent and non-adherent HAM phi populations.

US$29.90

PMID: 6486733


Other references

Ozols, Y.N.; Roga, I.F., 1973: Study of dielectric properties of milk using resonance methods. Milk was placed in a coaxial cell in a resonant circuit, operating at frequencies in the range 40-140 MHz, and the change of frequency of resonance was observed. A difficulty was experienced because of the high conductivity of the milk but this wa...

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Noebels, J.L., 2005: Calcium channel "gaiting" and absence epilepsy. Epilepsy Currents 5(3): 95-97

Anonymous, 1953: DISEASES notified in each state and territory of Australia for the week ended May 9, 1953. Medical Journal of Australia 1(24): 867-867

Jan Meulenbelt, 2012: Chlorine. The clinical symptoms appear instantly on exposure and may consist of nasal discharge, lacrimation, pain in the upper airways, dyspnoea, bronchospasm, bronchial oedema, oedema of the glottis, increased mucus production, haemoptysis and cyanosis.

Saxena, S.; Singh, T.; Agarwal, H.; Mehta, G.; Dutta, R., 2011: Bacterial colonization of rings and cell phones carried by health-care providers: are these mobile bacterial zoos in the hospital?. Our objective was to assess the presence of pathogenic organisms on the rings (worn on fingers) and cell phones carried by health-care workers (HCWs) and the public. Forty-two percent of mobile phones carried by HCWs and 18% carried by the general...

Dunlop, J.; Gardiner, S., 1993: Phosphate uptake, proton extrusion and membrane electropotentials of phosphorus-deficient Trifolium repens L. Trifolium repens plants were grown to produce a range of levels of phosphate stress. The most severely stressed plants absorbed phosphate at twice the rate for plants grown in adequate phosphate. Characteristics of phosphate uptake, membrane elect...

Nilipour, A.H.; Pierson, E.E.M.; Knight, C.D.; Dong, J., 1998: Effect of a novel hatchling supplement on performance of light weight chicks held for 25 hours prior to farm placement. Poultry Science 77(SUPPL 1): 42

Ischebeck, T.; Zbierzak, A.Maria.; Kanwischer, M.; Dörmann, P., 2005: A salvage pathway for phytol metabolism in Arabidopsis. Chlorophyll is the most abundant photosynthetic pigment in higher plants. During senescence, chlorophyll is hydrolyzed, resulting in the release of free phytol and chlorophyllide. Although the degradation of chlorophyllide has been studied in dept...

Jirout, J., 1973: February 1948 and pharmacies. Ceskoslovenska Farmacie 22(2): 49-52