+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A random arrangement of albumin-containing hepatocytes seen with histo-immunologic methods. I. Verification of the artifact

, : A random arrangement of albumin-containing hepatocytes seen with histo-immunologic methods. I. Verification of the artifact. Anatomical Record 197(2): 183-194

The cellular and subcellular localization of albumin in hepatocytes of adult male rats was established with immunofluorescence and immunoperoxidase techniques. Livers were fixed while either filled or devoid of blood. In some rats, prior treatment with cycloheximide was used to deplete the albumin content of hepatocytes. Immunofluorescence of blood-free livers from untreated rats showed that all hepatocytes contained albumin. However, using the peroxidase method, the amount of immunoprecipitate in cisternae of the rough endoplasmic reticulum was so slight that specific localization of albumin was impossible. Yet in all cases, a positive reaction for the presence of albumin was seen on ribosomes attached to the endoplasmic reticulum. In contrast, immunofluorescence of blood-filled livers from untreated rats and those previously injected with cycloheximide showed that only a few scattered hepatocytes were positive for albumin. In these cases, subcellular localization of albumin was obvious because the immunoprecipitate was found in heavy concentration, buy only in the cytosol compartment.


PMID: 6998323

DOI: 10.1002/ar.1091970207

Other references

Flagg, J.M., 1982: Consultation in community residences for the chronically mentally ill. Journal of Psychosocial Nursing and Mental Health Services 20(12): 30-35

Lian, J.B.; Tassinari, M.; Glowacki, J., 1984: Resorption of implanted bone prepared from normal and warfarin-treated rats. Bone that was virtually depleted of the vitamin K-dependent protein, osteocalcin, and 93% reduced in the concentration of its characteristic amino acid, gamma-carboxyglutamic acid, was obtained from rats treated with warfarin for 6 wk. Osteocalcin...

Vrzal, E.M.; Allan, S.A.; Hahn, D.A., 2010: Amino acids in nectar enhance longevity of female Culex quinquefasciatus mosquitoes. Culex mosquitoes feed on a wide range of nectars consisting of mostly carbohydrates and amino acids, however, little is known about the utilization and effects of these different carbohydrates and their accompanying amino acids on longevity. Culex...

Malik, M.A.; Maharaj, C.H.; Harte, B.H.; Laffey, J.G., 2008: Comparison of Macintosh, Truview EVO2, Glidescope, and Airwayscope laryngoscope use in patients with cervical spine immobilization. The purpose of this study was to evaluate the effectiveness of the Pentax AWS, Glidescope, and the Truview EVO2, in comparison with the Macintosh laryngoscope, when performing tracheal intubation in patients with neck immobilization using manual i...

Murray, B.C.; Belton, M.J.; Danielson, G.E.; Davies, M.E.; Gault, D.E.; Hapke, B.; O'leary, B.; Strom, R.G.; Suomi, V.; Trask, N., 1974: Mercury's Surface: Preliminary Description and Interpretation from Mariner 10 Pictures. The surface morphology and optical properties of Mercury resemble those of the moon in remarkable detail and record a very similar sequence of events. Chemical and mineralogical similarity of the outer layers of Mercury and the moon is implied; Me...

Mikesell, Jan E., 1994: Growth enhancement induced by herbivory in Pharbitis nil Choisy. American Journal of Botany 81(6 SUPPL ): 29

Alfano, Flora, 1994: Effects of the lichen Cladonia foliacea on the development of protonemata from spores and shoots in Bryum sp. Allelopatic interactions between bryophytes and lichens are studied. The protonema of the moss Bryum sp. from spores or shoots represents our study system. The different development patterns of the protonemal apparatus grown in culture medium (H-2...

Gill S.A., 1980: Design and construction of rock caissons. Pages 241-252 1980

Teh, B., S.; Mai, W.-Yuan; Grant, W., H.-Iii; Chiu, J.K.m; Lu, H., H.; Carpenter, L.S.even; Woo, S., Y.; Butler, E.B.ian, 2002: Intensity modulated radiotherapy (IMRT) decreases treatment-related morbidity and potentially enhances tumor control. Intensity modulated radiation therapy (IMRT), a new form of three-dimensional conformal radiation therapy (3DCRT), optimizes the concept of computer-controlled radiation deposition in tumor (target) while sparing adjacent normal structures. A retr...

Prol Ledesma R.M.; Taran Y.A.; Inguaggiato S., 2003: Geochemistry of fluids from submarine hot springs at Punta de Mita, Nayarit, Mexico; discussion and reply. Journal of Volcanology and Geothermal Research 121(3-4): 313-322