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A rapid, centrifugation-free radioimmunoassay specific for human chorionic gonadotropin using glass beads as solid phase


, : A rapid, centrifugation-free radioimmunoassay specific for human chorionic gonadotropin using glass beads as solid phase. Journal of Clinical Endocrinology and Metabolism 50(1): 169-175

A simple, specific, rapid, centrifugation-free solid phase RIA for hCG has been developed. Purified gamma-globulin isolated from antisera produced against hCG-beta in rabbits is covalently linked to glutaraldehyde-activated aminopropyl glass beads. The entire assay can be completed within 3 h at room temperature. The sensitivity (1 ng/ml), precision (intra- and interassay variabilities, less than 10% and less than 15%, respectively), and specificity (0.4% cross-reactivity with LH) are comparable to other methods. The hCG levels determined by the present method show good correlation with those obtained by another RIA and by radioreceptorassay. The present method also provides the basis for the development of similar rapid and sensitive assays of other hormones.

US$19.90

PMID: 7350180

DOI: 10.1210/jcem-50-1-169


Other references

Oliveira, M.S.N.; Ni, X.W.i, 2004: Effect of hydrodynamics on mass transfer in a gas-liquid oscillatory baffled column. In this paper, we present an experimental study on mass transfer of oxygen into water in an oscillatory baffled column (OBC). The objective of this work is to establish the contribution of individual parameters, such as the size of bubbles and the...

Mason, A.; Wick, M.; White, H.; Perrillo, R., 1993: Hepatitis B virus replication in diverse cell types during chronic hepatitis B virus infection. Hepatitis B virus-specific nucleic acid sequences and proteins have been detected in extrahepatic tissues of acutely and chronically infected patients. However, apart from peripheral blood mononuclear cells and bone marrow cells, little is known a...

Cauvin, F., 1952: Protection of the pregnant in Morocco. Maroc Medical 31(330): 997-998

Mason C.F.; Elliot K.L.; Clelland S., 1987: Landscape change in a parish in essex eastern england uk since 1838. Landscape planners and wildlife conservationists have placed great stress on the loss of habitat on farmland since the 1950's. However, there is little information on habitat loss prior to the 1950's, although it is generally considered...

Foulkes, A.J.; Barkley, D.; Biktashev, V.N.; Biktasheva, I.V., 2011: Alternative stable scroll waves and conversion of autowave turbulence. Rotating spiral and scroll waves (vortices) are investigated in the FitzHugh-Nagumo model of excitable media. The focus is on a parameter region in which there exists bistability between alternative stable vortices with distinct periods. Response...

Basu, A.; Ballantyne, J.M., 1979: Random fluctuations in first-order waveguide grating filters. A simple method is proposed for studying the spectral response of a first-order waveguide grating filter with random fluctuations in the period of the grating. The method can be applied to any grating groove shape, and it can be used to study the...

Laugero, L.A.; Oberbauer, A.M., 2000: Stability of alternatively spliced IGF-I mRNA in growth plate chondrocytes. Insulin-like growth factor-I (IGF-I) enhances the proliferation and hypertrophy of growth plate chondrocytes leading to increased growth plate width thus promoting bone elongation. Differing quantities of the multiple IGF-I transcripts within the...

Niiya, Y.; Crocker, J.; Bartmess, E.N., 2004: From vulnerability to resilience: learning orientations buffer contingent self-esteem from failure. An experiment examined the buffering effects of a learning orientation following failure in a domain of contingent self-worth. Participants' academic contingencies of self-worth (CSW) and priming with theories of intelligence interacted to af...

Jeffress, R.N.; Peter, J.B.; Lamb, D.R., 1968: Effects of exercise on glycogen synthetase in red and white skeletal muscle. Life Sciences 7(18): 957-960

Suzuki, 1986: Spin property of heavy hadron in heavy-quark fragmentation: A simple model. Physical Review. D, Particles and Fields 33(3): 676-682