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A sensitive colorimetric assay for various proteases using naphthyl ester derivatives as substrates


, : A sensitive colorimetric assay for various proteases using naphthyl ester derivatives as substrates. Journal of Biochemistry 87(3): 779-783

A convenient and highly sensitive colorimetric assay for various proteases, such as trypsin, chymotrypsin, plasmin, thrombin, and urokinase is described. The substrates used are alpha-naphthyl ester derivatives of N alpha-tosyl-L-lysine, N alpha-acetylglycyl-L-ination of alpha-naphthol released from them. Use of these alpha-naphthyl ester derivatives made the method more sensitive than the use of the corresponding methyl or ethyl ester derivatives. The minimum detectable concentrations of trypsin, chymotrypsin, plasmin, thrombin and urokinase in this method were about 0.002 micrograms, 0.01 microgram, 0.002 CU, 0.01 IU, and 2 IU, respectively. The Km values of trypsin and thrombin for TLNE were 0.11 mM and 0.15 mM while those for TLME were 2.5 mM and 6.7 mM, respectively; the Km values of chymotrypsin for ATNE and ATEE were 0.18 mM and 0.7 mM, respectively; and the Km values of urokinase for AGLNE and AGLME were 0.17 mM and 4 mM, respectively. Zymograms of various proteases were easy to prepare using these alpha-naphthyl ester substrates, and zymograms of trypsin and chymotrypsin were made with TLNE and ATNE, respectively, as substrates.

US$29.90

PMID: 6446549


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Hinrichs, K.; Spensley, M.S.; Mcdonough, P.L., 1992: Evaluation of progesterone treatment to create a model for equine endometritis. To investigate a model for equine endometritis, 12 mares with normal reproductive tracts were divided into 2 groups. All mares received progesterone in oil, 250 mg im, daily. At 5 days after initiation of progesterone administration, the uteri wer...

Matsuzaki, M.; Sugawara, K.; Adachi, K.; Hongo, S.; Nishimura, H.; Kitame, F.; Nakamura, K., 1992: Location of neutralizing epitopes on the hemagglutinin-esterase protein of influenza C virus. Neutralization-resistant variants of influenza C/Ann Arbor/1/50 virus were selected with monoclonal antibodies against four different antigenic sites on the hemagglutinin-esterase (HE) glycoprotein, and their HE genes were sequenced to identify am...

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Moses, D.F.; D.L.s Heras, M.A.; Valcarcel, A.; Perez, L.; Baldassarre, H., 1995: Use of computerized motility analyzer for the evaluation of frozen-thawed ram spermatozoa. A computerized motility analyzer (CellTrak/S) was calibrated for its use with ram semen. Adjustment of the several setup variables allowed an accurate measurement of kinetic parameters such as percentage of motile cells, straight velocity, curvili...

Sharer, L.R.; Michaels, J.; Murphey-Corb, M.; Hu, F.S.; Kuebler, D.J.; Martin, L.N.; Baskin, G.B., 1991: Serial pathogenesis study of SIV brain infection. A serial study of early SIV brain infection revealed initial involvement of leptomeninges, followed by perivascular infection of brain parenchyma. Severity of SIV encephalitis correlated with severity of systemic disease rather than with length of...

Kasabov, R.; Nikiforova, M., 1982: Extraction and lyophilization of avian herpesvirus strain SZ-1 of turkey origin. A live vaccine for immunizing fowls against Marek's disease was prepared by treating cultures of turkey herpesvirus in duck embryo fibroblasts with ultrasound. Similar results were obtained with three protective media for lyophilization: 5% p...

Costanzo, G.; D.M.uro, E.; Negri, R.; Pereira, G.; Hollenberg, C., 1995: Multiple overlapping positions of nucleosomes with single in vivo rotational setting in the Hansenula polymorpha RNA polymerase II MOX promoter. In vivo nucleotide-level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is reported. The 4 nucleosomes analyzed are organized in families; they localize in alternative positions along a...

Leone B.J.; Spahn D.R., 1992: Prophylaxis and treatment of ischemia. International Anesthesiology Clinics 30(1): 155-176

Sahni, V.M.; Davis, R.L., 1958: Self-incompatibility studies in alfalfa. Two self-incompatible diploid Medicago sativa plants were used in a study of the inheritance of this character. On the basis of the results obtained from a considerable number of crosses, a compatibility system is proposed in which at least two lo...

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