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A sensitive double isotope modification of the Farr assay using beta-particle emitters


, : A sensitive double isotope modification of the Farr assay using beta-particle emitters. Journal of Immunoassay 4(1): 99-109

A sensitive modification of the ammonium sulfate precipitation assay for antibody affinity is described. The assay combines small reaction volumes and a 32P volume marker to determine the average relative affinity of small amounts of antibody to DNP hapten. The assay is relatively safe since two beta-particle emitters are utilized instead of two gamma-ray emitters. The assay has proven useful for the determination of small amounts of antibody such as might be found in secretions and column fractions.

US$29.90

PMID: 6853728


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