geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive dual wavelength microspectrophotometer for the measurement of tissue fluorescence and reflectance


, : A sensitive dual wavelength microspectrophotometer for the measurement of tissue fluorescence and reflectance. Pflugers Archiv 385(2): 167-173

The construction of a microscope photometer using prefabricated elements is described. To illuminate the tissue, a Leitz Ultropac is applied. To enlarge the wavelength range, its illuminating glass lens is replaced by an Acryl glass zonal lens. Two separate light channels with separate lamps, monochromators and photomultipliers allow the measurement of fluorescence excitation and emission spectra as well as of reflection spectra. By chopping the light, light pulses and dark currents are measured 8.33 times a second. By an integration circuit the signal-to-noise ratio for small signals is improved. The instrument allows us to detect the increase of 4 ng/ml NADH (solution of pH 7.38) in an area of 0.2 mm2.

US$29.90

PMID: 7190276


Other references

Riabkova, Ls, 1974: Pollen morphology of Scapicephalus Ovcz. et Czuk and taxons nearest to it. Doklady 7 (9) 67-69

Qadri, S.S.H., 1972: An assay to study the effect of texture of soil surfaces on the availability of insecticides of insects. In laboratory tests in Warangal, India, endrin, heptachlor, toxaphene and DDT were applied in sprays at 0.354 and 0.708 mg/cm2 to three types of soil in shallow dishes, and adults of Musca domestica nebulo F. were kept in contact with the treated...

Etcheverry, M., 1989: Los 'Anales Agronomicos'. Revista Chilena de Historia Natural, 621: 149-156

Thomas, D K. ., 1984: Gower birds 1983. Systematic list. Gower Birds, 42: 3-47

Seo, N.Sook.; Han, M.Sook.; Lee, J.Soon., 2006: Effects of a tilting training program on lower extremities function, depression, and self-efficacy among stroke inpatients. The purpose of this study was to identify effects of a tilting training program on lower extremities function, depression, and self-efficacy among stroke inpatients. The subjects were 60 stroke patients hospitalized in an oriental medical hospital...

Kern, K.G., 1961: On increment measurements with the Arnberg microdendrometer.. Describes hourly variations of diameter readings at b.h. on three 55-year and two ca. 110-year Spruce trees on various days during the growing season, and discusses the effect of meteorological factors on them. On clear days with dry air, when tra...

Akchurin, A.; Saginbaev, A.; Igubaev, A., 1979: Evaluation and forecast of the suitability of tractor engines to the diagnosis. Vestnik sel'skokhoziaistvennoi nauki Kazakhstana: (2) 88-94

Meyerholz, Gw, 1981: Future of dairy herd in hands of person rearing calves. Hoard's dairyman, 126(19): 1311

Herridge, D.F.; Atkins, C.A.; Pate, J.S.; Rainbird, R.M., 1978: Allantoin and allantoic acid in the nitrogen economy of the cowpea vigna unguiculata. The ureides, allantoin and allantoic acid, represented major fractions of the soluble N pool of nodulated plants of cowpea (V. unguiculata [L.] Walp. cv. Caloona) throughout vegetative and reproductive growth. Stem and petioles were the principal...

Taylor, S.J.; Bartlett, M.J.; Greenfield, S.A., 1988: Release of acetylcholinesterase within the guinea-pig substantia nigra: effects of 5-hydroxytryptamine and amphetamine. A soluble form of acetylcholinesterase is released from the substantia nigra of the cat, rabbit and rat. The possibility of spontaneous and evoked release of acetylcholinesterase within the substantia nigra of a different species, the guinea-pig,...