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A sensitive enzyme immunoassay system for the measurement of beta-thromboglobulin in plasma and urine

, : A sensitive enzyme immunoassay system for the measurement of beta-thromboglobulin in plasma and urine. Clinica Chimica Acta; International Journal of Clinical Chemistry 123(1-2): 111-119

A sensitive enzyme immunoassay system for the measurement of beta-thromboglobulin (beta-TG) in plasma and urine was developed using purified antibodies to beta-TG linked to beta-D-galactosidase from Escherichia coli as label. The assay system consisted of polystyrene beads with immobilized antibody F(ab')2 fragments and the antibody Fab' fragments labeled with beta-D-galactosidase. The assay had a sensitivity of 10 pg/assay tube. beta-TG levels in plasma and urine could be determined with 1-2.5 microliters and 100 microliters samples, respectively. The results obtained with the enzyme immunoassay correlated well with those of a radioimmunoassay (r = 0.946, slope = 0.846, y-intercept = 4.71 ng/ml; n = 60). Plasma beta-TG levels in healthy subjects determined with the present assay system were 38.4 +/- 12.2 ng/ml (+/- 1 SD). Levels were markedly increased in 19 out of 22 patients with myeloproliferative syndrome. Urinary beta-TG levels in healthy subjects were estimated to be 0.17 +/- 0.05 ng/ml (+/- 1 SD). Urine levels were increased in 16 out of the 19 patients with myeloproliferative syndrome whose plasma levels were high. These findings indicate that urinary beta-TG levels may be reflection of the beta-TG in plasma and can be an indicator of platelet activation.


PMID: 6180847

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Chong, K.Yee.; Liu, H.; Yin, K.; Harrison, P.J.; Kau, K.Kei., 2017: A bottom water sampler for determining chemical gradients across the water-sediment interface. We developed an inexpensive bottom water sampler that can be operated from a small boat in order to obtain high resolution vertical profiles of dissolved oxygen (DO), chlorophyll, suspended particulate matter (SPM). These vertical profiles allow u...

Kaplan, M.E.; Zalusky, R.; Remington, J.; Herbert, V., 1962: Immunological studies with intrinsic factor in man. Jour Clin Invest 41(6): 1370

Crossen, P.E.; Morgan, W.F., 1979: Proliferation of PHA-stimulated lymphocytes measured by combined autoradiography sister chromatid differential staining. Experimental Cell Research 118(2): 423-426

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Rao G.S.; Krishna G.; Gillette J.R., 1974: Covalent binding of aromatic amino and nitro compounds to rat liver microsomal proteins. Pharmacologist 16(2): 277

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Wrate R.M.; Rooney A.C.; Thomas P.F.; Cox J.L., 1985: Postnatal depression and child development a 3 year follow up study. This study investigates whether 3-yr-old children whose mothers were depressed after their birth showed more behavior disturbance than children of mothers who were not depressed at that time. Of 103 mothers who took part in an earlier prospective...