+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive enzyme-linked immunosorbent assay for the quantitation of antigen-specific murine immunoglobulin E

, : A sensitive enzyme-linked immunosorbent assay for the quantitation of antigen-specific murine immunoglobulin E. Journal of Immunological Methods 45(1): 51-63

An enzyme-linked immunosorbent assay (ELISA) for the quantitation of 2,4-dinitrophenyl (DNP)-specific murine immunoglobulin (Ig) E is described. The assay uses beta-galactosidase, which is conjugated to goat anti-rabbit gamma-globulin (GARG) via a method using a mild heterobifunctional cross-linking reagent, m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). The assay has a sensitivity for detection of about 200 pg/ml of anti-DNP IgE. Analyses of murine serum samples using this ELISA correlate well with those obtained using the passive cutaneous anaphylaxis (PCA) reaction in rats. With the use of automated 96-well reader, data acquisition is rapid and, therefore, this ELISA is ideal for analyses of large numbers of samples. The assay can be easily modified for the measurement of other Ig classes and of IgE of other antigen specificities.


PMID: 6169764

Other references

Alcazar-Bejerano, I.Lynne., 2014: Health Behaviors, Disparities and Deterring Factors for Breast Cancer Screening of Immigrant Women - A Challenge to Health Care Professionals. This literature review was made to provide comprehensive to provide comprehensive understanding of health disparities as well as factors and barriers to cancer screening of immigrant women in multicultural societies. Published articles from 1990-2...

Hunter, O.B., 1952: DR. Oscar B. Hunter. Southern Medical Journal 45(2): 164-164

Wang-Ying-Yong; Cui-Rong-Feng, 2007: A new avian subspecies record in mainland China, Ficedula narcissina owstoni (Passeriformes, Muscicapidae). A first-spring male of Ficedula narcissima owstoni was recorded in the Bamboo Arboretum of Sun Yat-sen University in Haizhu Borough, Guangzhou, on 7 Apr. 2005. Photos were taken as proof. This is a new avian subspecies record in mainland China. A...

Vechernin V.V.; Kovalev O.V., 1988: New mathematical model of the solitary wave for description of population wave. A new phenomenon of forming solitary population wave of insects (Zygogramma suturalis, Chrysomelidae) appearing after their introduction into a free ecological niche was discovered. During the settling of insects in a new area as a result of purpo...

Zhang, X.L.; Yang, G.H.; Li, R.Y.; Hu, Y.Z.; Zhuang, Z.F., 1987: Primary malignant fibrous histiocytoma of bone. Clinico-pathologic analysis of 45 cases. Hua Xi Yi Ke Da Xue Xue Bao 18(2): 179-182

Khrustaleva, A.M.; Pavlov, D.A., 2000: Peculiarities of otolith morphology in some fish species form from the White Sea. Voprosy Ikhtiologii 40.5 (Sentyabr-Oktyabr): 655-667

Poruban Steven, 2002: East Mediterranean natural gas prospects, projects hit pay dirt. Oil and Gas Journal 100(20): 20-23

R.Stephen J.S.arks; James G.M.ore; Carl J.R.ce, 1986: The initial giant umbrella cloud of the May 18th, 1980, explosive eruption of Mount St. Helens. The initial eruption column of May 18th, 1980 reached nearly 30 km altitude and released 1017 joules of thermal energy into the atmosphere in only a few minutes. Ascent of the cloud resulted in forced intrusion of a giant umbrella-shaped cloud bet...

Koizumi, S.; Tsuda, M.; Shigemoto, Y.; Obama, T.; Inoue, K., 2001: Characterization of P2Y receptors in cultured rat dorsal root ganglion neurons. Japanese Journal of Pharmacology 85(Supplement 1): 149P

Johns, D.W.; Blum, D., 1993: Complex partial seizures without surface EEG changes during ictal recording. Epilepsia 34(SUPPL 6): 130