+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive enzyme-linked immunosorbent assay of serum ornithine carbamoyltransferase

, : A sensitive enzyme-linked immunosorbent assay of serum ornithine carbamoyltransferase. Enzyme & Protein 48(1): 10-17

Ornithine carbamoyltransferase (OCT), a urea cycle enzyme, is located almost exclusively in liver mitochondria. We designed a sensitive enzyme-linked immunosorbent assay (ELISA) of serum OCT protein using an antibody against purified bovine enzyme. OCT protein measured using this method showed a good correlation with OCT activity (r = 0.961), and was much higher in patients with liver diseases than in the controls. Measurements of serum OCT protein in 442 healthy blood donors gave the upper limit of normal range of 23 ng Eq/ml (equivalent to the bovine enzyme) for males, 8 ng Eq/ml for females, and 16 ng Eq/ml for males plus females. The values differ significantly between the sexes and depending on ages. This ELISA system is expected to be used as a pertinent liver function test.


PMID: 7787966

Other references

Dillavou, E.D.; Cohn, H.E., 2003: Minimally invasive parathyroidectomy: 101 consecutive cases from a single surgeon. Intraoperative rapid parathyroid hormone (iPTH) assay is changing parathyroid surgery. One surgeon's experience at a tertiary care hospital was followed as minimally invasive parathyroidectomy (MIP) was adopted. In this prospective case study...

Klemens, S.P.; DeStefano, M.S.; Cynamon, M.H., 1992: Activity of clarithromycin against Mycobacterium avium complex infection in beige mice. The activity of clarithromycin alone and in combination with other antimycobacterial agents was evaluated in the beige (C57BL/6J bgj/bgj) mouse model of disseminated Mycobacterium avium complex (MAC) infection. A dose-response experiment was perfo...

Bertuso J.R.; Flaker G.C.; Ruder M.A.; Alpert M.A., 1984: Do patients with cardiac arrest and hypokalemia require antiarrhythmic drug therapy?. American Heart Association Monograph (107): II-443

Xie GuanLin; Soad, A.; Swings, J.; Mew, T.W., 2003: Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment. With the use of a seed washing technique, more than 4000 Gram negative bacteria were isolated by two improved isolation methods from 446 batches of 1 kg rice seed samples obtained from 22 provinces in the Philippines. They were initially character...

Chirkin, V.V., 1969: Overcoming tissue incompatibility in homoplastic skin grafting in sexually mature rats. Biulleten' Eksperimental'noi Biologii i Meditsiny 67(5): 80-82

Kallersjo M., 1985: Fruit structure and generic delimitation of athanasia asteraceae anthemideae and related south african genera. Within South African Asteraceae-Anthemideae there is a group of genera containing furanosesquiterpenes rather than the common polyacetylenes. Of these genera, Asaemia (Harv.) Harv. ex Benth. and Hook., Athanasia L., Eumorphia DC., Gymnopentzia Ben...

Kovas, A.E.; McFarland, B.H.; McCarty, D.J.; Boverman, J.F.; Thayer, J.A., 2007: Buprenorphine for acute heroin detoxification: diffusion of research into practice. Buprenorphinc has been approved for heroin detoxification, but little is known about its impact on everyday practice. Concerns about buprenorphine include expense, limited knowledge about its use, patient limits, and social and clinical attitudes...

Endress P.K., 1985: Stamen abscission and pollen presentation in annonaceae. In certain Annonaeceae, in the male phase of the protogynous flowers, the abscising stamens remain attached to the torus by extended spiral thickenings of their vascular bundle. They provide a loosening of the compact androceium and allow better p...

Stromberg, W.B.; Ackley, W.O., 1953: Unusual acute abdomens. Illinois Medical Journal 104(3): 213-214

Brolemann, H.W., 1932: Lysiopetalide (Myriapode-Diplopode) caveraicole nouveau recueilli en Greca par M. le Dr. Georges Blanc, Directeur de l'Institut Pasteur du Maroc. Bulletin de la Societe Zoologique de France T: 57(No. 1: 45-53