+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive fluorimetric rate assay for biotinidase using a new derivative of biotin, biotinyl-6-aminoquinoline

, : A sensitive fluorimetric rate assay for biotinidase using a new derivative of biotin, biotinyl-6-aminoquinoline. Analytical Biochemistry 140(1): 69-73

The previously reported method for the estimation of biotinidase (EC is an endpoint colorimetric assay based on the hydrolysis of biotinyl-4-aminobenzoate, followed by diazotization, and is not suitable for our studies of biotinidase. A fluorimetric rate assay of biotinidase which uses a newly synthesized derivative biotinyl-6-aminoquinoline is described here.


PMID: 6548338

Other references

Rossi, N.; Nucciotti, F., 1968: Chemical composition of atmospheric precipitation in the Emilia regions. I. Sulphur. Amounts of S supplied to the soil by rainfall varied from an annual 112 kg/ha (near industrial cities) to 14 kg, and averaged 17-23 kg in many agricultural regions.

Javidanpour, S.; Fatemi Tabtabaei, S.Reza.; Siahpoosh, A.; Morovati, H.; Shahriari, A., 2012: Comparison of the effects of fresh leaf and peel extracts of walnut (Juglans regia L.) on blood glucose and β-cells of streptozotocin-induced diabetic rats. There is some report about the hypoglycemic effect of Juglans rejia L. leaf in alloxan induced diabetic rats and hypoglycemic effect of its fruit peel administered intra peritoneally. Thirty male Wistar rats divided into five groups, to evaluate t...

Greene, W.C.; Fleisher, T.A.; Waldmann, T.A., 1981: Soluble suppressor supernatants elaborated by concanavalin a activated human mononuclear cells 1. characterization of a soluble suppressor of t cell proliferation. When activated with the mitogenic lectin, concanavalin A, a human peripheral blood mononuclear cell subset, differentiates into potent suppressor cells that negatively modulate both cellular and humoral immune reactions. In addition to inhibitory...

Higby, D.J.; Cohen, E.; Holland, J.F.; Sinks, L., 1974: The prophylactic treatment of thrombocytopenic leukemic patients with platelets: a double blind study. Transfusion 14(5): 440-446

Waisbrod, N.; Buchbinder, E.; Possick, C., 2012: In-home intervention with families in distress: changing places to promote change. This article examines the benefits of in-home family therapy with severely distressed families through the analysis of four cases that demonstrate the creative use of this intervention with families whose children were placed in a full-time day ca...

Beharka, A.A.; Nagaraja, T.G.; Morrill, J.L.; Kennedy, G.A.; Klemm, R.D., 1998: Effects of form of the diet on anatomical, microbial, and fermentative development of the rumen of neonatal calves. Eight neonatal, Holstein bull calves were paired by birth date and birth weight and randomly assigned to either a finely ground or unground control diet (chopped hay and rolled grain) to study the effects of the physical form of the diet on anatom...

Vyhovs'-Kyi, Oo, 1978: Ways of increasing production ef. Lisove hospodarstvo lisova paperova i derevoobrobna promyslovist': (2) 8-9

Kelly, E., 1950: Riboflavin and niacin content of green, snap beans. Bountiful and Green Pod Stringless green snap beans contained, respectively, per 100 g., an average over 3 years of 0.65 and 0.66 mg. nicotinic acid and 0.125 and 0.124 mg. riboflavin. About 14 per cent. riboflavin and 20 per cent. nicotinic acid...

Ergün, Y., 2005: Differential effects of nifedipine and verapamil on non-adrenergic non-cholinergic relaxations at different levels of active tone: assessment of the contribution of nerve-derived hyperpolarizing factor. 1 The objective was to investigate a possible contribution of a nerve-derived hyperpolarizing factor to the differences between non-adrenergic non-cholinergic (NANC) nerve-mediated relaxations in different states of active tone in the rat gastric...

Catherine, N.; Giovangrandi, Y.; Bazin, A.; Daffos, F.; Forestier, F., 1991: Failure of fetal karyotyping and diagnosis of complete Di George syndrome. Lancet 337(8736): 299-300