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A sensitive immunoenzymometric assay for 2',5'-oligoadenylate. Detection of elevated 2',5'-oligoadenylate synthetase in human peripheral mononuclear cells


, : A sensitive immunoenzymometric assay for 2',5'-oligoadenylate. Detection of elevated 2',5'-oligoadenylate synthetase in human peripheral mononuclear cells. Journal of Immunological Methods 65(1-2): 123-135

A competition immunoenzymometric assay for 2',5'-oligoadenylate was developed and employed to measure the interferon-inducible enzyme 2',5'-oligoadenylate synthetase in cell extracts. Microtiter plates coated with a novel conjugate of p5'A2'p5'A2'p5'A and N-(2-aminoethyl)-carbamylmethylated-Ficoll (AECM-Ficoll) bound rabbit polyclonal or mouse monoclonal antibody directed against 2',5'-oligoadenylate. Binding was inhibited by soluble 2',5'-oligoadenylate. Estimates of 2',5'-oligoadenylate concentrations based on inhibition of antibody binding compared favorably with those obtained using a protein synthesis inhibition assay. Low concentrations of 2',5'-oligoadenylate synthesized in vitro by extracts of human peripheral mononuclear cells were conveniently estimated using less than or equal to 10(6) cells. Virtually identical results were obtained when either total extract or synthetase bound to poly(I) . poly(C)-agarose was used for the in vitro incubation. When peripheral mononuclear cells were incubated in vitro with interferon, the normally low levels of 2',5'-oligo(A) synthetase rose dramatically. The assay was employed to measure synthetase levels in peripheral mononuclear cells isolated from patients with systemic lupus erythematosus. Some of these patients were found to have elevated levels of 2',5'-oligoadenylate synthetase.

US$29.90

PMID: 6361140


Other references

Tarshis, I.B.rry; Blinstrub, R., 1963: Preliminary studies on the use of sorptive dusts for the control of the human lice, Phthlrus pubis and Pediculus humanus capitis De Geer. The sorptive dusts, Dri-Die 67, 2% Dibrom-Dri-Die 67, and 0.05% Pyrethrum-Dri-Die 67 have been found effective against pubic lice, Phthirus pubis and the sorptive dust 2% Dibrom-Dri-Die 67 has been found effective against head lice, Pediculus huma...

Richter, HJ., 1971: Ein Zwergcichlide mit Temperament. Nannochromis dimidiatus Pellegrin 1900. Aquar terrar, 18: 148-150

Lee YoungWon, 1999: Transcolonic scintigraphy for diagnosis of canine portosystemic shunts. Transcolonic scintigraphy using 99mTechnetium pertechnetate (4TcO99m) was performed in 5 dogs with portosystemic shunts. Radioactivity in the heart was seen earlier than in the liver. The mean shunt index in 5 dogs was 82.3% (range 79.6-87.1%). It...

Ruppert, F.; Rottgardt, A., 1926: A New Method of preparing Foot and Mouth Disease Serum and its Effectiveness. According to the authors the virus of foot-and-mouth disease remains localized at the point of entry and does not gain the blood stream until five hours have elapsed. They hold that the period of infectivity of the blood is in reality longer than...

L.V.ctor C.F.; Rice James R., 1980: Time-dependent tectonic scale earthquake instability model for a strike-slip boundary. Eos, Transactions, American Geophysical Union 61(46): 1050-1051

Anonymous, 1966: Annual report of the Department of Animal Husbandry for the year 1965. During 1965 the estimated milk production in Cyprus was cows' milk, 13, 500 tons; ewes' milk, 15, 000 tons and goats' milk, 16, 000 tons and the estimated number of animals in milk was 4, 500 cows, 260, 000 ewes and 150, 000 goats....

Offenbacher, S.; Kline, E.S., 1984: Evidence for polyphosphate in phosphorylated nonhistone nuclear proteins. Structural studies of eucaryotic nuclear proteins have revealed the presence of bound polymeric phosphates. 32P-labeled and nonlabeled nonhistone nuclear proteins (NHPs) were isolated from rat liver nuclei and subjected to various controlled hydro...

Li, J.; Wang, J.; Li, J.; Liu, D.; Li, H.; Gao, W.; Li, J.; Liu, S., 2016: Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch. In the present study, the culture conditions for the accumulation of Glycyrrhiza uralensis adventitious root metabolites in balloon-type bubble bioreactors (BTBBs) have been optimized. The results of the culture showed that the best culture condit...

Silani, V.; Scarlato, G., 1993: Molecular biology of neurotrophic factors in the central nervous system. Polli, E E [Editor] International Congress Series; Molecular bases of human diseases 199-206

Koplay, M.; Keser Emiroglu, M.; Cimen, D.; Soylu, H., 2015: Rosary-like giant coronary artery aneurysms in Kawasaki disease: Diagnosis with prospectively ECG-gated dual source CT angiography. Revista Clinica Espanola 216(1): 47-47