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A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

, : A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS. Brain Research 298(2): 235-241

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.


PMID: 6202368

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