geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive method for determination of serum hemoglobin based on iso-luminol chemiluminescence


, : A sensitive method for determination of serum hemoglobin based on iso-luminol chemiluminescence. Clinica Chimica Acta; International Journal of Clinical Chemistry 122(2): 125-133

A simple and rapid method for determination of serum hemoglobin is described. Hemoglobin may be determined in serum within the range of 0.02-400 mg/1 by the sensitive chemiluminescent iso-luminol reaction. The iso-luminol assay was considerably more sensitive than the conventional colorimetric procedure based on tetramethylbenzidine. Precision and accuracy were higher with the iso-luminol assay especially at low levels of hemoglobin. The correlation between the luminescent and colorimetric method was linear but the colorimetric determinations resulted in higher concentrations of hemoglobin. This discrepancy was probably caused by non-heme serum iron which interfered more strongly with the colorimetric method.

US$29.90

PMID: 7105405


Other references

Yang, W.T.; Daly, B.D.; Li, E.K.; Hutchinson, R., 1993: Cranial computed tomography in the assessment of neurological complications in critically ill patients with systemic lupus erythematosus. The cranial computed tomography findings in 22 critically ill patients with systemic lupus erythematosus in the intensive care unit were reviewed to document the spectrum of pathology encountered and to assess the contribution of cranial computed...

Mazzullo Salvatore J., 1985: Pennsylvanian facies-diagenetic reservoir, lower Strawn Formation, Seminole Southeast Field, Midland Basin, West Texas. Pages 227-238 1985

Hotz, C.; Gibson, R.S., 2007: Traditional food-processing and preparation practices to enhance the bioavailability of micronutrients in plant-based diets. Dietary quality is an important limiting factor to adequate nutrition in many resource-poor settings. One aspect of dietary quality with respect to adequacy of micronutrient intakes is bioavailability. Several traditional household food-processin...

Germs G.J.B., 1968: Discovery of a new fossil in the nama system southwest africa fossil namalia villiersiensis new genus new species archaeocyatha sponges. Nature (London) 219(5149): 53-55

Josiah, D.T.; Boo, S.; Tarabishy, A.; Bhatia, S., 2016: Anatomical differences in patients with lumbosacral transitional vertebrae and implications for minimally invasive spine surgery. NlmCategory="UNASSIGNED">OBJECTIVE The objective of this study was to investigate the neurovascular and anatomical differences in patients with lumbosacral transitional vertebrae (LSTV) and the associated risk of neurovascular injury...

Ednick, M., D.; Pagala, M.; Barakat, J.-Pierre; Nino, G.; Shah, P.; Cunningham, J., N.; Vaynblat, M.; Kazachkov, M., 2007: Telemetric recording of intrapieural pressure. Background. Monitoring of intrapleural pressure (IPP) is used for evaluation of lung function in a number of pathophysiological. conditions. We describe a telemetric method of non-invasive monitoring of the IPP in conscious animals intermittently...

Nishikawa H.; Fujii H., 1986: Physiological applications of nmr and esr imaging. Biomedical Research (Tokyo) 7(SUPPL 2): 85-88

Spelman, D.; Harrington, G.; Russo, P.; Wesselingh, S., 2002: Clinical, microbiological, and economic benefit of a change in antibiotic prophylaxis for cardiac surgery. Vancomycin and rifampicin replaced cephazolin as antibiotic prophylaxis for coronary artery bypass surgery at our institution. Following this intervention, there was a significant decrease (P < .001) in the surgical-site infection rate from 10....

Rubin, B.; Wegener, A.M.K.; Liabeuf, N.; Jorgensen, A.W., 1990: Biological transfer of the CBA Tcra locus into C57BL/6 mice. Breeding experiments are described in which the T-cell receptor alpha locus (Tcra) was backcrossed to the C57BL/6 strain to create Tcra congenic mice. Using restriction fragment length polymorphism markers for Tcra genes, it was found that no reco...

Brzozowski, A., 1971: Evaluation of utilisation of pasture for feeding heifers. In 1969 in Poland 60 replacement Friesian heifers 1 to 2 years old grazed on a pasture on peat land. The pasture produced 3614 oat feed units (FU)/ha, and on average 89% of the herbage was eaten. Heifers gained from 130 to 750 g daily and required...