+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive method for the assay of oxytocin in the circulating blood

, : A sensitive method for the assay of oxytocin in the circulating blood. British Journal of Pharmacology 52(2): 295-305

1 The sensitivity of a wide range of isolated tissues to oxytocin was investigated. The longitudinal muscle strip of duck pulmonary vein proved the most suitable tissue for use with the superfusion technique, contracting to concentrations to oxytocin as low as 10 muu/ml.2 The duck pulmonary vein superfused with Krebs solution was contracted by oxytocin, the vasopressins, adrenaline, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II. Pre-treatment of the preparation with phenoxybenzamine (1-2 mug/ml) abolished the contractions to catecholamines and reduced the effects of histamine and 5-hydroxytryptamine without affecting the sensitivity to oxytocin.3 The pulmonary vein contracted when superfused with blood from an anaesthetized dog. This contraction was accompanied by a non-specific loss of responsiveness. When the pulmonary vein was superfused with Krebs solution that had been dialysed against blood the initial contraction was greatly reduced or abolished as was the loss in responsiveness.4 Oxytocin was stable in circulating dog's blood but approximately 50% was bound to plasma proteins. Oxytocin was not destroyed in the pulmonary circulation or the hind limbs. In the doses used oxytocin had a half-life of 60-90 s in the circulation of the dog under steady-state conditions. Disappearance occurred chiefly in the visceral vascular beds.


PMID: 4451824

Other references

Segal, T.; Gordin, V.; Novy, D., 2004: A lucrative routine, short on pain management. Pain Medicine 3(4): 349; Discussion 349-50

Clark, R.M.; Zera, A.J.; Behmer, S.T., 2015: Nutritional physiology of life-history trade-offs: how food protein-carbohydrate content influences life-history traits in the wing-polymorphic cricket Gryllus firmus. Although life-history trade-offs result from the differential acquisition and allocation of nutritional resources to competing physiological functions, many aspects of this topic remain poorly understood. Wing-polymorphic insects, which possess al...

Won, G-Jone.; Fudge, D.S.; Choh, V., 2015: The effects of actomyosin disruptors on the mechanical integrity of the avian crystalline lens. Actin and myosin within the crystalline lens maintain the structural integrity of lens fiber cells and form a hexagonal lattice cradling the posterior surface of the lens. The actomyosin network was pharmacologically disrupted to examine the effec...

Ramdohr P., 1936: Bertrandit von Klein-Spitzkopje, Suedwestafrika. Describes the secondary beryllium mineral bertrandite from its first known African occurrence, Klein- Spitzkopje, South-West Africa.

Wallis J.R., 1982: Hydrologic problems associated with oilshale development. Pages 85-102:

Diskin, C.J., 1989: AIDS-related complex by continuous ambulatory peritoneal dialysis. Nephron 51(4): 548-548

Braynion, P., 2011: "Person-centred care is led by learning disability nurses". Nursing Times 107(32-33): 11-11

Lee, A.Pui-Wai.; Acker, M.; Kubo, S.H.; Bolling, S.F.; Park, S.W.; Bruce, C.J.; Oh, J.K., 2009: Mechanisms of recurrent functional mitral regurgitation after mitral valve repair in nonischemic dilated cardiomyopathy: importance of distal anterior leaflet tethering. Recurrent functional mitral regurgitation (MR) has been reported after mitral valve repair with annuloplasty in patients with dilated cardiomyopathy, but the mechanism is not understood completely. The authors sought to identify abnormalities of t...

Diaz-Perez, R.; Quiroz, H.; Nishiyama, R.H., 1976: Primary mucinous adenocarcinoma of thyroid gland. A mucin-producing carcinoma in the thyroid gland found in a 44-year-old man was first thought to be a metastatic carcinoma, possibly from salivary gland. However, follow-up examinations for 8 years have not demonstrated another neoplasm, and it se...

Thomas G.M., 1990: Management of early stages of seminoma. Journal of Cancer Research & Clinical Oncology 116(SUPPL PART 2): 951