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A sensitive method for the detection and isolation of recombinants of foot-and-mouth disease virus

, : A sensitive method for the detection and isolation of recombinants of foot-and-mouth disease virus. Journal of General Virology 53(Pt 2): 333-342

Recombination between temperature-sensitive (ts) mutants of foot-and-mouth disease (FMD) virus was examined, using an infectious centre technique that was more sensitive (approx. 30-fold) than the conventional virus yield test. The test involved a brief incubation of the mixedly infected cells at the permissive temperature to allow recombination to occur followed by assay at the restrictive temperature to select for those cells in which recombination had occurred. With crosses involving widely separated mutations, as many as 28% of the infected cells produced presumptive recombinant plaques. Since each plaque was the result of an independent event, large numbers of different presumptive recombinants could be isolated for further study. Analysis of presumptive recombinant plaques from a variety of crosses showed that, in general, the virus produced had the properties expected of recombinants. An approximate correlation was found between genetic distance, as determined in the yield recombination test, and the percentage of recombinant infectious centres observed. The phenomenon was very sensitive to the balance between the input multiplicities of the two parent viruses and occurred very early in virus replication. The test has considerable potential for the study of genetic interactions in FMD virus, but it would be surprising if this potential was limited to picornaviruses.


PMID: 6267181

DOI: 10.1099/0022-1317-53-2-333

Other references

Piccinni, M.P.; Macchia, D.; Parronchi, P.; Giudizi, M.G.; Bani, D.; Alterini, R.; Grossi, A.; Ricci, M.; Maggi, E.; Romagnani, S., 1991: Human bone marrow non-B, non-T cells produce interleukin 4 in response to cross-linkage of Fc epsilon and Fc gamma receptors. Human bone marrow (BM) cells lacking T- and B-cell markers expressed RNA encoding interleukin (IL) 4 and secreted detectable amounts of IL-4 in supernatants in response to Fc epsilon or Fc gamma receptor (Fc epsilon R or Fc gamma R) cross-linking....

Werneck A.M.; Gontijo Filho P.P., 1983: Gas liquid chromatography quantification of heptadecane and hexadecane degradation by mycobacteria. GLC was performed to analyze the ability of 4 strains of M. fortuitum and 1 of M. triviale isolated from soil and sewage to degrade haptadecane, hexadecane, diesel fuel and petroleum. This assay allowed the quantification of hydrocarbon consumptio...

Graddon, Wd, 1972: Some new discomycete species. 2. Transactions of the British Mycological Society 58(1): 0-159

Tsurusawa, M.; Watanabe, T.; Gosho, M.; Mori, T.; Mitsui, T.; Sunami, S.; Kobayashi, R.; Fukano, R.; Tanaka, F.; Fujita, N.; Inada, H.; Sekimizu, M.; Koh, K.; Kosaka, Y.; Komada, Y.; Saito, A.M.; Nakazawa, A.; Horibe, K., 2015: Randomized study of granulocyte colony stimulating factor for childhood B-cell non-Hodgkin lymphoma: a report from the Japanese pediatric leukemia/lymphoma study group B-NHL03 study. The objective of this study was to assess the impact of the primary prophylaxis of granulocyte colony-stimulating factor (G-CSF) in the management of childhood B-cell non-Hodgkin lymphoma (B-NHL). Patients with advanced-stage mature B-NHL were ran...

Anonymous, 1979: Brown planthopper: threat to rice production in Asia. In May 1977, a symposium was held at the International Rice Research Institute in the Philippines to discuss the results of research on Nilaparvata lugens (Stal) on rice and to develop plans for its control. The objectives of the symposium were to...

Kadar, I.; Nemeth, T., 2003: Studies on the leaching of microelement pollutants in a long-term field experiment. A small-plot microelement pollution field experiment was set up in spring 1991 on a calcareous chernozem soil with loam texture formed on loess, at the Nagyhorcsok Experimental Station of the Research Institute for Soil Science and Agricultural Ch...

Haines, R.W., 1942: Eudiarthrodial joints in fishes. Journal of Anatomy 77(Pt 1): 12-19

Joyner W.B.; Porter L.D.; Perez V., 1979: Strong-motion records from the Coyote Lake earthquake, California. Eos, Transactions, American Geophysical Union 60(46): 889

Anonymous, 1991: Chlorinated drinking-water. Iarc Monographs on the Evaluation of Carcinogenic Risks to Humans 52: 45-141

Motta, G., 1988: ET nurse entrepreneurs. Journal of Enterostomal Therapy 15(5): 181-182