+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive method for the routine determination of plasma nicotine by flame ionization gas-liquid chromatography

, : A sensitive method for the routine determination of plasma nicotine by flame ionization gas-liquid chromatography. Analytical Biochemistry 143(2): 316-319

A gas chromatographic procedure for the analysis of nicotine in plasma, which uses quinoline as an internal standard, is reported. The nicotine is extracted with diethyl ether, concentrated without any evaporation, thus avoiding losses, and analyzed without derivatization. The recovery is 83.2 +/- 6.1% (n = 6). Although the analysis is carried out with a classical flame ionization detector, the detection limit is 0.1 ng/ml. Linearity is observed up to 100 ng/ml. The results of the precision analysis performed in the working range indicate a good reproducibility: a coefficient of variation of 5.2% is obtained for within-run analysis and 10.5 to 4.5% for nicotine values from 2.9 to 19.1 ng/ml for day analysis. Since a single run (the limitative step) lasts less than 15 min this improved procedure allows a great number of samples to be processed per day.


PMID: 6532246

Other references

Medem, A.V.; Seidling, H.M.; Eichler, H-Georg.; Kaltschmidt, J.; Metzner, M.; Hubert, C.M.; Czock, D.; Haefeli, W.E., 2017: Definition of variables required for comprehensive description of drug dosage and clinical pharmacokinetics. Electronic clinical decision support systems (CDSS) require drug information that can be processed by computers. The goal of this project was to determine and evaluate a compilation of variables that comprehensively capture the information contain...

Westerholm, R.; Christensen, A.; Tornqvist, M.; Ehrenberg, L.; Rannug, U.; Sjogren, M.; Rafter, J.; Soontjens, C.; Almen, J.; Gragg, K., 2001: Comparison of exhaust emissions from Swedish environmental classified diesel fuel and European Program on Emissions, Fuels and Engine Technologies reference fuel A chemical and biological characterization, with viewpoints on cancer risk. Diesel fuels, classified as environmentally friendly, have been available on the Swedish market since 1991. The Swedish diesel fuel classification is based upon the specification of selected fuel composition and physical properties to reduce poten...

Alexopoulos, E.I.; Kaditis, A.G.; Kostadima, E.; Gourgoulianis, K., 2005: Resolution of nocturnal enuresis in snoring children after treatment with nasal budesonide. Nocturnal enuresis has been associated with obstructive sleep apnea-hypopnea and may resolve after adenotonsillectomy. Nasal corticosteroids have improved symptoms and polysomnography findings in children with snoring. Two children with primary no...

Arieli A.; Werner D., 1989: A comparison between fermentation heat of forages and organic matter digestibility determined by in vitro incubation with rumen fluid. The potential of calorimetry as a method for the estimation of energy availability in ruminant feeds was studied. The in vitro fermentation heat of feeds and their in vitro organiic matter digestibility (IVOMD) were determined in separate experime...

Saitoh, H.; Kobayashi, N.; Maekawa, T., 1986: Turnover study of prothrombin, antithrombin III and fibrinogen in patients with liver cirrhosis. In twenty patients with liver cirrhosis and eleven healthy volunteers, the turnover of prothrombin, antithrombin III (AT III) and fibrinogen was investigated. In liver cirrhotic patients, blood levels of various clotting factors were lowered, with...

Jenden D.J., 1972: Simultaneous micro estimation of choline and acetyl choline by gas chromatography mass spectrometry isotope dilution. Federation Proceedings 31(2): 515

Singh Gurdev, 1989: Quaternary geology and geomorphology of parts of Bhatinda, Faudkot and Ferozepur districts, Punjab; B, Mansa area, Bhatinda District. Records of the Geological Survey of India 122, Part 8: 55-59

Matsson, M.; Hederstedt, L.; Cochran, B.; Ackrell, B.A.C., 1997: Carboxin resistance in Paracoccus denitrificans conferred by a mutation in the membrane-anchor domain of succinate Quinone oxidoreductase. FASEB Journal 11(9): A859

Wallace, J.J., 1944: The use of wood in aircraft design and construction. Part II. Some of the problems involved in the design and construction of wooden aircraft, using flat or moulded plywood, are discussed. The advantages gained by using fluid pressure techniques are said to have been exaggerated. By using carefully selected...

Drees, Michael., 2000: Records of the apterous small cicada Errhomenus brachypterus near Hagen-Hohenlimburg Homoptera Cicadellidae Nachweise der flugunfahigen Kleinzikade Errhomenus brachypterus bei Hagen-Hohenlimburg Homoptera Cicadellidae. Decheniana. August; 153: 263