geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive new method for measurement of guanase with 8-azaguanine in bicine bis-hydroxy ethyl glycine buffer as substrate


, : A sensitive new method for measurement of guanase with 8-azaguanine in bicine bis-hydroxy ethyl glycine buffer as substrate. Clinica Chimica Acta; International Journal of Clinical Chemistry 115(2): 135-144

Serum guanase activity has been considered as a possible specific indicator of hepatocellular diseases. However, no suitable method is available for routine clinical determination of serum guanase activity. Conventional assay methods are troublesome and inaccurate, since guanine and 8-azaguanine, the substrates of the enzyme, are scarcely soluble in water so that it is not possible to prepare a stable substrate solution of sufficient concentration for use in assays. A new method was developed for assay of guanase activity by direct colorimetric determination of ammonia. In this method, bicine bis-hydroxy ethyl glycine (dotite bicine) buffer is used for preparation of a stable substrate solution and with a fixed concentration of substrate of sufficient strength serum guanase can be measured sensitively and reproducibly. This assay system could be used as a routine clinical laboratory test in the diagnosis of liver damage.

US$29.90

PMID: 7285360


Other references

Singh, J.P.; Chaikin, M.A.; Stiles, C.D., 1982: Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay. Competition between 125I-labeled platelet-derived growth factor (PDGF) and unlabeled PDGF forms the basis of a specific "radio-receptor assay" for quantifying PDGF in clotted blood serum. Human clotted blood serum contains 15 ng/ml of PD...

Hannah, J.; Kahn, S., 1989: The relationship of socioeconomic status and gender to the occupational choices of grade 12 students. The purpose of the present study was to investigate the socioeconomic status (SES) background and gender of adolescents in relation to the prestige level and gender composition of occupations. In Part 1 of the study, Gottfredson's (1981, Jour...

Bax, G.; Lelli, S.; Grandis, U.; Cospite, A.M.; Paolo, N.; Fedele, D., 1995: Early involvement of central nervous system in type I diabetic patients. To investigate whether the asymptomatic involvement of the central and peripheral nervous systems may be an early complication of diabetes. We studied early impairment of the central and peripheral nervous system pathways in 15 type I diabetic pat...

Séguin, J.R.; Beigbeder, J.Y.; Hvass, U.; Langlois, J.; Grolleau, R.; Jourdan, M.; Klein, B.; Bataille, R.; Chaptal, P.A., 1992: Interleukin 6 production by cardiac myxomas may explain constitutional symptoms. Journal of Thoracic and Cardiovascular Surgery 103(3): 599-600

Liu, Z.D.; Liu, D.Y.; Lu, S.L.; Hider, R.C., 2000: Design, synthesis, and biological evaluation of aromatic ester prodrugs of 1- -2-methyl-3-hydroxypyridin-4-one as orally active iron chelators. In order to improve chelation efficacy and to minimise toxicity, eleven aromatic ester prodrugs of 1-(3'-hydroxypropyl)-2-methyl-3-hydroxypyridin-4-one (CP41) have been synthesised. The distribution coefficients of these ester prodrugs betwee...

Tarocco, C.; Giannini, A., 1989: Backfat thickness in the sow at farrowing and weaning and the weaning - heat interval (preliminary report). In a sample of 78 sows backfat thickness at weaning and the difference between this variable at farrowing and at weaning were found not to be valid indications in predicting the onset of heat within or after 7 days of weaning. Condition scoring an...

Lundquist Gary M.; Samowitz Ilene R., 1982: Relative attenuation properties for 12 paths about the eastern Kazakh Test Site. Eos, Transactions, American Geophysical Union 63(45): 1038-1039

Lowndes Ashley G., 1954: A first tracer mineral. Suggests that an investigation of the distribution of sanidine containing nosean inclusions in bottom sediments of British coastal waters might provide significant information on the character and direction of bottom currents. The nosean is a mino...

Torra R., 2000: Geochemical signatures in littoral shallow marine deposits (Miocene), northeastern Argentina. International Geological Congress, Abstracts = Congres Geologique International, Resumes 31: (unpaginated

Anonymous, 2013: Engineering of sperm production. Sperm production is a key male reproductive trait and an important parameter in sperm competition theory. Under sperm competition, paternity success is predicted to depend strongly on male allocation to sperm production. Furthermore, because sperm...