geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive radioenzymatic assay for dihydroxymandelic acid, dihydroxypenylglycol and dihydroxyphenylacetic acid


, : A sensitive radioenzymatic assay for dihydroxymandelic acid, dihydroxypenylglycol and dihydroxyphenylacetic acid. Journal of Biochemical and Biophysical Methods 4(5-6): 261-270

A sensitive radioenzymatic procedure for the simultaneous determination of dihydroxymandelic acid, dihydroxyphenylglycol, and dihydroxyphenylacetic acid has been developed. The catechols were methylated by catechol-O-methyltransferase using S-adenosyl[methyl-3H]methionine as methyl donor. The assay involved extraction into organic solvent, thin-layer chromatography and periodate oxidation. The applicability of the procedure was tested by measuring the amounts of deaminated metabolites of noradrenaline, adrenaline and dopamine in heart perfusion samples and plasma of rabbits.

US$29.90

PMID: 7019296


Other references

Amissi, S.; Boisramé-Helms, J.; Burban, Mélanie.; Rashid, S.K.; León-González, A.J.; Auger, C.; Toti, F.; Meziani, F.; Schini-Kerth, Vérie.B., 2017: Lipid Emulsions Containing Medium Chain Triacylglycerols Blunt Bradykinin-Induced Endothelium-Dependent Relaxation in Porcine Coronary Artery Rings. Lipid emulsions for parenteral nutrition are used to provide calories and essential fatty acids for patients. They have been associated with hypertriglyceridemia, hypercholesterolemia, and metabolic stress, which may promote the development of end...

Neely, D., A.; Perez, M., H.S.baj; Mendsaikhan, B., 2008: First records of Rhinogobius lindbergi Teleostei Gobiidae and Abbottina rivularis Teleostei Cyprinidae in the Lake Buyr drainage, Mongolia. We report the first records of Amur goby, Rhinogobius lindbergi and the Chinese false gudgeon, Abbottina rivularis from the Lake Buyr drainage of eastern Mongolia. Both species were previously known only from much farther downstream in the Amur R...

Tsugawa, N.; Yamabe, T.; Takeuchi, A.; Kamao, M.; Nakagawa, K.; Nishijima, K.; Okano, T., 1999: Intestinal absorption of calcium from calcium ascorbate in rats. The intestinal absorption of calcium (Ca) from Ca ascorbate (Ca-AsA) was investigated in normal rats. Each animal was perorally administered either 5mg (low dose) or 10mg (high dose) of Ca in 1ml of distilled water as Ca-AsA, Ca carbonate (CaCO3),...

Wu, Y-T.; Wu, Y-W.; Hwang, C-L.; Wang, S-S., 2013: Changes in diastolic function after exercise training in patients with and without diabetes mellitus after coronary artery bypass surgery. A randomized controlled trial. Left ventricular diastolic dysfunction and diabetes were associated with prognosis after coronary artery bypass surgery (CABG). This study investigated whether short-term exercise improves diastolic function in patients with and without diabetes m...

Ilan, B.; Sivan, Y.; Fibich, G., 2011: A quantitative approach to soliton instability. We present an approach for instabilities of solitons that is based on the spectrum of a fourth-order linearized operator. Unlike the standard approach which is based on the slope (Vakhitov-Kolokolov) condition, this approach provides the quantitat...

Baylis, H.A., 1937: On the ascarids parasitic in seals, with special reference to the genus Contracaecum. Baylis reviews 9 species of ascarid worms which have been obtained from seals. He is of the opinion that Ascaris rectangula and A. stenocephala are synonyms of Contracaecum osculum, a full description of which species he gives. C. radiatum is also...

Keung, Y.K.; Mallarino, M.C.; Cobos, E., 1998: Drug-induced Evans syndrome. Annals of Internal Medicine 128(4): 327-327

Gersting, Søren.W.; Lotz-Havla, A.S.; Muntau, A.C., 2012: Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells. In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular functio...

Miyasaka, K.; Kitani, K., 1986: Effects of bile salts on pancreatic secretion in rabbits: ursodeoxycholate infused into the duodenum stimulates pancreas. The effects of six species of bile salts, deoxycholate, ursodeoxycholate, glycodeoxycholate, tauroursodeoxycholate, chenodeoxycholate, and cholate (DCA, UDCA, GDC, TUDC, CDCA, CA), on pancreatic secretion were examined in anesthetized rabbits. Whe...

Green, M.J., 1987: Electrochemical immunoassays. Immunoassays are routinely used to detect, specifically, low levels of many antigens. The trend away from the use of radioisotopic labels has resulted in a proliferation of alternative labels, many of which have electrochemical activity. The more...