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A sensitive radioimmuno assay for thymine dimers

, : A sensitive radioimmuno assay for thymine dimers. Molecular & General Genetics 186(4): 475-477

A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers (TT) has been developed. The limit of detection of this method is 6 X 10(-14) mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer (UU) interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.


PMID: 6957705

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