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A sensitive radioimmunoassay for bovine anti-Müllerian hormone, allowing its detection in male and freemartin fetal serum


, : A sensitive radioimmunoassay for bovine anti-Müllerian hormone, allowing its detection in male and freemartin fetal serum. Endocrinology 111(4): 1409-1411

Two monoclonal antibodies were used to set up a solid-phase RIA [radioimmunoassay] for bovine anti-Mullerian hormone (bAMH). One AMH unit is defined as the amount released by 1 g of bovine fetal testicular tissue during a 4-h incubation period. Calibration curves were prepared using aliquots of a standard 500-ml pool of incubation medium, containing 200 AMH mU/ml, diluted either in 50% pig testes incubation medium, 5% horse serum, 10% female calf fetal serum or pure female calf fetal serum. Linearization of the calibration curves was achieved through logit-log transformation; all 4 lines were parallel. Within- and between-assay variability were less than 5%. The RIA is at least 600 times more sensitive than the bioassay for anti-Muellerian activity and can detect AMH in male and freemartin fetal serum.

US$19.90

PMID: 6896853

DOI: 10.1210/endo-111-4-1409


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