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A sensitive radioimmunoassay for quantitation of IgM rheumatoid factor


, : A sensitive radioimmunoassay for quantitation of IgM rheumatoid factor. Arthritis and Rheumatism 23(3): 302-308

A solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgM rheumatoid factor (RF) in biologic fluids has been developed. Binding curves for monoclonal IgM RF and polyclonal IgM rheumatoid factors were similar under the conditions utilized for the assay. Human IgG did not interfere with the detection of IgM RF by this method. Small quantities (less than or equal to 0.2%) of nonspecific binding by nonRF IgM to the human IgG coated tubes utilized in the assay were corrected for by assaying samples in parallel bovine serum albumin coated control tubes. As expected, patients with seropositive rheumatoid arthritis (RA) had significantly higher concentrations of IgM RF than seronegative RA patients (mean +/- 1 SD = 652 +/- 553 microgram/ml versus 11.3 +/- 13.3 microgram/ml, P less than 0.001). In contrast, all normal control sera assayed to date contained less than 0.1 microgram/ml of IgM RF. The capacity of the assay to detect nanogram quantities of IgM RF should permit investigation of the cellular mechanisms underlying RF production.

US$29.90

PMID: 7362681


Other references

Potvin, P.J.; Schutz, R.W., 2000: Statistical power for the two-factor repeated measures ANOVA. Determining a priori power for univariate repeated measures (RM) ANOVA designs with two or more within-subjects factors that have different correlational patterns between the factors is currently difficult due to the unavailability of accurate met...

Timoshechkina, M.E.; Budilina, G.I., 1964: Results Of The Combined Use Of Sarcolysin And Brain Tissue Emulsion Under Experimental Conditions. Biulleten' Eksperimental'noi Biologii i Meditsiny 58: 88-90

Tang, J.Q.; Béné, M.C.; Faure, G.C., 1991: Alternative rearrangements of immunoglobulin light chain genes in human leukemia. Immunoglobulin heavy and light chain genes undergo rearrangement before they can be expressed by B-cells. A sequence of successive rearrangements has been proposed, suggesting that these events are initiated on heavy chain genes and are followed b...

Bogdanov, M.B.; Beal, M.F.int; Mccabe, D.R.; Griffin, R.M.; Matson, W.R., 1999: A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2'-deoxyguanosine measurements in urine and other biologic matrices: A one-year evaluation of methods. 8-Hydroxy-2'-deoxyguanosine (8OH2'dG) is a principal stable marker of hydroxyl radical damage to DNA. It has been related to a wide variety of disorders and environmental insults, and has been proposed as a useful systematic marker of ox...

Miller M.J.S.; Westlin W.F.; Carroll M.A.; Mcneill H.; Mcgiff J.C., 1985: Compartmental prostaglandin release from isolated perfused kidneys of normotensive and hypertensive rabbits. Clinical Research 33(2 PART 1): 365A

Flick, B.; Klug, S., 2006: Whole embryo culture: an important tool in developmental toxicology today. The number of candidate chemicals or drugs for registration and authorization is increasing at a fast rate and only few of the existing substances have been tested for teratogenicity to date. Therefore, there is high pressure on authorities to acc...

Swienty, W., 1939: Die Blutgefasse der Bauchflossen mancher Teleosteer (Salmo, Barbus). Bull Acad Cracovie B: 11: 51-59

Zhang, X.H.; Shen, H.M.n; Englert, W., 1993: Two new species of the genus Tarsonemus from Germany (Acari: Tarsonemidae). Acta Zootaxonomica Sinica ; 18(4): 424-428

Scully, C.; Malamos, D., 2014: Clinical challenges Q&A. 1. Sore gums. Dental Update 41(5): 471-472

Newell, R.S., 1989: Post-Soviet Afghanistan: the position of minorities. Having maintained their dominance for over two centuries despite endemic rivalries among themselves, the Pushtuns now face an unprecedented challenge to their supremacy with the departure of the Soviet forces. Virtually all mujahideen organization...