+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive radiometric assay for enkephalin convertase and other carboxypeptidase B-like enzymes

, : A sensitive radiometric assay for enkephalin convertase and other carboxypeptidase B-like enzymes. Life Sciences 34(2): 113-121

A sensitive radiometric assay for carboxypeptidase B-like enzymes has been developed using enkephalin convertase, an enkephalin synthesizing carboxypeptidase. The assay is based on the differential solubility of 3H-labeled substrate and product in chloroform. The substrates 3H-benzoyl-Phe-Ala-Arg or 3H-benzoyl-Phe-Leu-Arg are poorly soluble in chloroform due to the charged arginine. The products of carboxypeptidase B-like activity on these substrates, 3H-benzoyl-Phe-Ala or 3H-benzoyl Phe-Leu partition quantitatively into chloroform, allowing rapid separation of product from substrate. This assay is approximately 100 times more sensitive than a similar fluorometric assay utilizing dansyl-Phe-Ala-Arg as a substrate.


PMID: 6420632

Other references

Hershberg, U.; Shlomchik, M.J., 2006: Differences in potential for amino acid change after mutation reveals distinct strategies for k and l light-chain variation. Proceedings of the National Academy of Sciences of the United States of America 103(43): 963-8

Hornby A.E.; Pan J.; Auersperg N., 1992: Intermediate filaments in rat ovarian surface epithelial cells changes with neoplastic progression in culture. Interrelationships between neoplastic progression and the expression of intermediate filaments were examined in primary cultures, immortal lines, and Kirsten murine sarcoma virus (KiMSV) transformed lines of rat ovarian surface epithelial (ROSE) c...

Baechler, M-Christine., 2013: "A little more love than usual". Krankenpflege. Soins Infirmiers 106(9): 60-62

Barry Nehring, B.; Schisler, G.; Chiaramonte, L.; Horton, A.; Poole, B., 2015: Assessment of the Long-Term Viability of the Myxospores of Myxobolus cerebralis as Determined by Production of the actinospores by Tubifex tubifex. While whirling disease was first observed in Rainbow Trout Oncorhynchus mykiss in 1893, the complete life cycle of Myxobolus cerebralis (Mc), the causative agent of the disease, was not understood until 1984, when it was shown to involve two oblig...

Dahlqvist, M., 1995: The significance of an across-shift decrease in vital capacity--a re-analysis of a study on subjects exposed to diesel exhaust. Occupational exposure to diesel exhaust may develop acute as well as chronic lung function impairment. In this study, data from an earlier study on a group of subjects working at tunnel construction site were analyzed. The aim of the analysis was...

Dare, J.G., 1953: Some quantitative studies on a bacterial pyrogen. A simple method giving a good yield of a highly active pyrogen extract from Proteus vulgaris and 2 different methods of measuring the pyrogenic effect on rabbits were used. It was found that body temp. increases with increasing doses. The minimal...

Jackson, W.T.; Hefzy, M.S.; Guo, H., 1994: Determination of wrist kinematics using a magnetic tracking device. The objective of this work is to present a method to determine the three-dimensional kinematics of the human wrist joint under physiological loading conditions using a magnetic tracking device. Euler angles were used to determine wrist extension-f...

Korenyi, C.; Whittier, J.R., 1969: Electroencephalographic study of dose-response relations for cyclandelate on hyperventilation-induced vasospasm in human brain. Angiology 20(9): 529-534

Palyusik, M.; Harrach, B.; Mirocha, C.J.; Pathre, S.V., 1979: Occurrence of zearalenol in the milk of sows fed zearalenone. Two milking sows consumed food containing 40 p.p.m. crystalline zearalenone for 9 days, starting 8 days after parturition. Their piglets were prevented from uptake of toxic feed. On the 4th-5th day piglets receiving sows' milk only started to...

Thrower, E.C.; Mobasheri, H.; Lea, E.J.A.; Dawson, A.P., 2000: Interaction of luminal calcium and cytosolic ATP in the control of type 1 Ins P3 receptor Study of channels reconstituted in planar lipid bilayers. Biophysical Journal 78(1 Part 2): 314A, Jan